摘要
目的掌握内蒙古包头市典型牧区环境中隐孢子虫污染情况。方法于2016年12月,采集包头市牧区土壤、牧草、饲料和饮用水、地表水、地下水、污水处理厂出厂水水样进行镜检,阳性样本利用巢式PCR扩增后进行基因测序,分析隐孢子虫种属特性。结果牛粪中隐孢子虫感染率为34.6%(72/208),水样隐孢子虫污染率为28.6%(2/7),土壤和植物样本均未检出隐孢子虫。4.2%(9/208)牛粪样本和28.6%(2/7)水样样本扩增出目标片段。测序比对结果显示11个PCR扩增阳性样本中隐孢子虫均为安氏隐孢子虫。结论包头地区牛粪及水样等环境中检出的隐孢子虫在种属上为安氏隐孢子虫,当地卫生部门应关注畜牧业对环境的影响。
Objective To know the pollution situation of Cryptosporidium in typical pastoral area of Baotou,Inner Mongolia.Methods In December 2016,the samples of soil,herbage,feed and drinking water,surface water,ground water and sewage treatment water were collected for microscopic examination.The positive samples were amplified by nested PCR and then sequenced to analyze the species characteristics of the Cryptosporidium.Results The rate of cattle infected with the Cryptosporidium was 34.6%(72/208),water sample was 28.6%(2/7),while none positive results were found in the soil sand plant samples.The positive rate of nested PCR amplification was 4.2%(9/208)in cow samples,two positive water samples were amplified and sequenced successfully.The results of sequencing showed that all the 11 positive samples were belonged to Cryptosporidium Andersoni.Conclusion Cryptosporidium Andersoni is the main cause of cryptosporidium contamination in the pasturing area of Baotou,and more attention should be paid to the animal husbandry related environmental impact.
作者
杨忠委
王华然
孙栋良
周树青
武月红
尹静
YANG Zhong-wei;WANG Hua-ran;SUN Dong-liang;ZHOU Shu-qing;WU Yue-hong;YIN Jing(Institute of Environmental and Operational Medicine,the Academy of Military Medical Sciences,Tianjin 300050,China;不详)
出处
《环境与健康杂志》
CAS
2024年第5期396-398,471,共4页
Journal of Environment and Health
基金
国家重点研发计划(2018YFC1602504,2017YFC1601206)
国家自然科学基金(31560655)
关键词
隐孢子虫
环境
水
牛粪便
Cryptosporidium
Environment
Water
Fecal
