摘要
目的建立血液和尿液中锂含量的电感耦合等离子体-质谱测定法。方法利用1%硝酸、0.1%曲拉通X-100-1%硝酸溶液分别对尿液和血液样品进行12.5、20倍直接稀释后上机检测,以钪(Sc)作为内标元素,于标准模式下完成检测。结果该方法利用标准曲线完成生物样品中锂含量测定,线性范围为0.03~50.0μg/L,相关系数为0.9999,方法的检出限为0.008μg/L,样品加标回收率为86.1%~103.8%,精密度为0.8%~4.2%,质量控制样品测定结果满意。结论本研究建立了一种简便快捷的测定生物样品中锂含量的方法,并对实验中各影响参数进行优化研究,详细探讨了实验过程中各参数变化对于实验结果的影响。方法前处理简便快捷、灵敏度高、稳定性好、检出限低,适用范围广,适合于各类人群生物样品锂含量的测定。
Objective Inductively coupled plasma-mass spectrometry was used to explore and study the influencing factors in the determination of lithium content in biological samples.It was established a simple and rapid method for determining the concentration of lithium ions in blood and urine.Methods Urine and blood samples were directly diluted 12.5 times/20 times using 1%nitric acid/0.1%triton X-100-1%nitric acid solution,scandium(Sc)was used as the internal standard element to complete the detection in standard mode.Results The method uses standard curve to determinate lithium content in biological samples,the linear range of this method was 0.03-50.0μg/L,the correlation coefficient was 0.9999,the detection limits were 0.008μg/L,the recoveries of samples were 86.1%-103.8%,the precisions were 0.8%-4.2%,quality control samples were measured satisfactorily.Conclusion A simple and rapid method for determining lithium content in biological samples were established in this study.The influencing parameters in the experiment are optimized and studied,and the influence of the changes of each parameter on the experimental results is discussed in detail.This method has the advantages of simple and fast operation,with high sensitivity,good stability,low detection limit and a wide range of application,which is suitable for the determination of lithium content of biological samples in various populations.
作者
杨柳
耿琪
姜开友
王超洋
秦文华
YANG Liu;GENG Qi;JIANG Kai-you;WANG Chao-yang;QIN Wen-hua(The Third People's Hospital of Henan Province(Henan Hospital for Occupational Diseases)Occupational and Environmental Testing and Inspection Center,Henan Provincial Key Laboratory of Chemical Toxicology Detection,Zhengzhou,Henan 450052,China)
出处
《环境与健康杂志》
CAS
2024年第1期67-70,95,共5页
Journal of Environment and Health
关键词
电感耦合等离子体-质谱法
锂
尿液
血液
Inductively coupled plasma-mass spectrometry
Lithium
Urine
Blood
