摘要
目的研究燃煤型氟中毒对仔鼠学习记忆能力的影响及其作用机制。方法将48只SD大鼠(雌鼠32只,雄鼠16只)按体重随机分为对照组、低氟组、中氟组、高氟组,饲料含氟量分别为5.2、24.4、47.8、106.0 mg/kg。雄鼠不染氟,雌鼠从青春期开始染氟至哺乳期,3个月后雌雄大鼠按2∶1合笼交配,仔鼠断乳后给予正常饲料。以Morris水迷宫检测仔鼠学习记忆能力,采用Western blot法检测仔鼠脑组织中神经型尼古丁受体(nAChRs)蛋白的相对表达水平,采用Pearson相关分析仔鼠脑组织nAChRs蛋白与学习记忆能力的相关性。结果成功复制氟中毒动物模型,氟中毒仔鼠学习记忆能力降低,差异有统计学意义(P<0.05);与对照组相比,各实验组仔鼠nAChR a7、a4和β2亚单位蛋白表达水平降低,差异有统计学意义(P<0.05),其中高氟组蛋白水平下降最明显。氟中毒仔鼠脑组织nAChR a7、a4、β2各亚型蛋白表达水平与穿越平台次数呈正相关(r值分别为0.391,0.454,0.462,P<0.05或P<0.01),与首次到达平台时间呈负相关(r值分别为-0.567,-0.564,-0.480,P<0.01),与逃避潜伏期呈负相关(r值分别为-0.612,-0.629,-0.570,P<0.01)。结论燃煤型氟中毒仔鼠学习记忆能力降低,与脑组织nAChRs蛋白表达水平有一定关系。
Objective To study the effect of coal-burning fluorosis on learning and memory ability of offspring rats and its mechanism.Methods Forty eight SD rats(32 females and 16 males)were randomly divided into control group,low fluoride group,medium fluoride group and high fluoride group.The fluoride contents in feed were 5.2 mg/kg,24.4 mg/kg,47.8 mg/kg and 106.0 mg/kg,respectively.The male rats were not exposed to fluoride,and the female rats began to be exposed to fluoride from puberty.After three months,the male and female rats were mated in cages with a ratio of 2∶1,and their offspring were fed with normal diet after weaning.Morris water maze was used to test the learning and memory ability of offspring rats.Western blot was used to detect the relative expression level of nicotine receptor protein in brain tissue of offspring rats.Pearson correlation analysis was used for correlation analysis.Results The learning and memory ability of offspring rats was decreased after successful establishment of fluorosis animal model;Compared with the control group,the protein expression levels of nAChR a7,a4 andβ2 subunit in fluoride exposure groups were decreased(P<0.05),and the protein levels of offspring rats in high fluoride group decreased significantly.The results of correlation analysis showed that nAChR a7,a4 andβ2 in brain tissue of offspring rats were significantly higher than those in control group(P<0.05).The protein levels of nAChR a7,a4 andβ2 in brain tissue of offspring rats in fluoride exposure groups were positively correlated with the times of crossing the platform(nAChR a7:r=0.391;nAChR a4:r=0.454;nAChRβ2:r=0.462;P<0.05 or P<0.01),negatively correlated with the time of first arriving at the platform(nAChR a7:r=-0.567;nAChR a4:r=-0.564;nAChRβ2:r=-0.480;P<0.01),and negatively correlated with escape latency(nAChR a7:r=-0.612;nAChR a4:r=-0.629;nAChRβ2:r=-0.570;P<0.01).Conclusion The decreased learning and memory ability of offspring rats with coal-burning fluorosis is related to the expression level of neural nicotine receptor protein in brain.
作者
王金玉
喻茂娟
贺娟
WANG Jin-yu;YU Mao-juan;HE Juan(Department of Epidemiology,School of Public Health,Guizhou Medical University,Guiyang,Guizhou 550025,China;不详)
出处
《环境与健康杂志》
CAS
北大核心
2020年第7期581-584,共4页
Journal of Environment and Health
基金
国家自然科学基金-贵州省人民政府联合基金(U1812403)
贵州医科大学神奇民族医药学院基金
关键词
燃煤型氟中毒
仔鼠
尼古丁受体
学习记忆能力
相关性分析
Coal burning fluorosis
Offspring rats
Nicotine receptor
Learning and memory ability
Correlation analysis
