摘要
选取35名无规律运动习惯的健康青年(年龄18~30周岁)分别完成一次急性高强度间歇训练(high intensity interval training,HIIT)和中等强度持续训练(moderate-intensity continuous training,MICT)(间隔一周),两种运动方式能量消耗相同(1256 kJ).分别于运动前、运动后即刻、运动后3 h和24 h采集上述青年的静脉血,采用流式细胞仪对低分化T细胞(CD3^(+)CD28^(+)CD57^(-))、高分化T细胞(CD3^(+)CD28^(-)CD57^(+))、调节性T细胞(T_(regs))(CD4^(+)CD25^(+)CD127^(-))、造血祖细胞(CD45^(+)CD34^(+))和内皮祖细胞(CD45^(-)CD34^(+)KDR^(+))检测计数;采用膜联蛋白V标记法检测T细胞及其亚群的凋亡率;采用电化学发光免疫分析法测定血清皮质醇含量;采用酶联免疫吸附法检测血清儿茶酚胺(肾上腺素和去甲肾上腺素)含量;采用荧光分光光度法检测血清硫代巴比妥酸反应物(TBARS)含量.结果显示:(1)运动后,两种运动方式均引起血清儿茶酚胺、皮质醇和TBARS含量升高(P<0.05);(2)运动后3 h,MICT诱导低分化T细胞凋亡率高于HIIT(P<0.05),运动后即刻,HIIT诱导的高分化T细胞凋亡率高于MICT(P<0.05);(3)运动后即刻和运动后3 h,HIIT方式的T_(regs)细胞计数高于MICT(P<0.05);运动后3 h,MICT方式的T_(regs)细胞凋亡率高于HIIT运动(P<0.05);(4)造血祖细胞和内皮祖细胞在不同运动方式间比较无显著性差异(P>0.05).以上结果表明,不同运动方式诱导的T淋巴细胞凋亡具有亚群特异性,其中HIIT诱导高分化T细胞凋亡,这可能造成机体对持续感染的免疫力暂时性下降,而MICT则诱导低分化T细胞和T_(regs)凋亡,进而可能暂时影响对新感染源的防御能力以及对炎症反应的调控.
To compare the changes of apoptosis in circulating T lymphocyte subsets during a bout of high-intensity interval training(HIIT)and moderate-intensity continuous training(MICT)and explore its possible mechanism,35 healthy youth with irregular exercise habits(aged 18to 30years old)completed an acute HIIT and MICT(an interval of a week)with the same energy consumption(1256kJ).Venous blood samples were collected before exercise,immediately after exercise,3hours and 24hours after exercise.The counts of low differentiated T cells(CD3^(+)CD28^(+)CD57^(-)),highly differentiated T cells(CD3^(+)CD28^(-)CD57^(+)),regulatory T cell(T_(regs))(CD4^(+)CD25^(+)CD127^(-)),hematopoietic progenitor cells(CD45^(+)CD34^(+))and endothelial progenitor cells(CD45^(-)CD34^(+)KDR^(+))were detected by flow cytometry.The apoptosis rate of T cells and their subsets was detected by Annexin V labeling.The content of serum cortisol was determined by electrochemiluminescence immunoassay,the content of serum catecholamine(epinephrine and norepinephrine)was determined by enzyme linked immunosorbent assay and the content of thiobarbituric acid-reactive substances(TBARS)was determined by fluorospectrophotometry.The results show that(1)both exercise modes increased the contents of serum catecholamine,cortisol and TBARS(P<0.05)after exercise;(2)the apoptosis rate of low differentiated T cells induced by MICT was higher than that of HIIT(P<0.05)at 3hafter exercise,whereas the apoptosis rate of highly differentiated T cells induced by HIIT was higher than that of MICT(P<0.05)immediately after exercise;(3)immediately after exercise and at 3hafter exercise,the number of T_(regs)cells in HIIT mode was higher than that in MICT(P<0.05),and the apoptosis rate of T_(regs)cells in MICT mode was higher than that in HIIT exercise(P<0.05)at 3hafter exercise;(4)there was no significant difference in hematopoietic progenitor cells and endothelial progenitor cells(P>0.05)between different exercise modes.The apoptosis of T lymphocytes induced by different exercise modes is subsets specific,in which HIIT induces apoptosis of highly differentiated T cells,which may temporarily decrease the immunity of the body to persistent infection,while MICT induces apoptosis of low differentiated T cells and T_(regs),which may temporarily affect defense against new infectious agents and regulation of inflammatory responses.
作者
贾钧
王晨宇
JIA Jun;WANG Chenyu(Department of Sports and Public Arts,Zhengzhou University of Aeronautics,Zhengzhou 450046,China)
出处
《河南大学学报（自然科学版）》
CAS
2023年第6期694-705,共12页
Journal of Henan University:Natural Science
基金
河南省重点研发与推广专项(科技攻关)项目(212102310266)
关键词
高强度间歇训练
中等强度持续训练
健康青年
免疫细胞
T淋巴细胞亚群
凋亡
激素
high intensity interval training
moderate-intensity continuous training
healthy youth
immune cells
T lymphocyte cell subsets
apoptosis
hormone
