摘要
【目的】研究苦瓜苗期耐热性遗传规律,为耐热苦瓜育种及相关分子生物学研究提供理论依据。【方法】对20份苦瓜自交系进行苗期耐热性评价,以耐热苦瓜自交系‘0974’、不耐热苦瓜自交系‘1590’为亲本,构建P1、P2、F1、F2、B1和B2共6个世代遗传群体,运用植物数量性状分离分析软件研究苦瓜苗期耐热性遗传规律。【结果】苦瓜苗期耐热性的遗传是受2对加性-显性-上位性主基因控制(B-1模型),控制苦瓜苗期耐热性的2对主基因加性效应分别为-0.846 8、-0.503 3,显性效应分别为0.099 2和-0.451 7,上位性效应以显性×显性互作效应为主,主基因遗传率在B1、B2、F2世代中分别为65.71%、61.20%、71.58%。【结论】分离材料应在早期世代进行人工定向选择,选择过程中要严格控制小区环境的一致性,降低环境影响。
【Objective】 A genetic inheritance analysis on the heat tolerance of bitter gourd at seedling stage was conducted for breeding and molecular biology studies. 【Method】 Heat tolerance of 20 inbred lines of bitter gourd at seedling stage was evaluated. Six generations(i.e., P1, P2, F1, F2, B1, and B2) of the cultivars were produced by hybridization and backcrossing between the heat-tolerant 0974 and the heat-sensitive 1590. From them, inheritability on heat tolerance of the plants was analyzed using a software for segregation analysis on quantitative traits. 【 Result】 In accordance to the B-1 model, the inheritance of the trait in bitter gourd at seedling stage appeared to be controlled by two major additive-dominance-epistasis pairs of genes. The additive effects by the two major gene pairs were-0.846 8 and-0.503 3, and the dominance effects 0.099 2 and-0.451 7;while the epistatic effect was mainly governed by dominant-dominant interaction. The rates of the inheritance in the B1, B2, and F2 generations were 65.71%, 61.2%, and 71.58%, respectively.【 Conclusion】 For effective bitter gourd breeding, the material segregation should be held in early generations, and the environmental conditions strictly controlled during the entire process to ensure consistent genetic transfer.
作者
陈中钐
许端祥
杜文丽
徐同伟
林碧英
高山
CHEN Zhongshan;XU Duanxiang;DU Wenli;XU Tongwei;LIN Bixing;GAO Shan(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian350002,China;Fuzhou Institute of Vegetable Crops,Fuzhou,Fujian350111,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2020年第7期731-738,共8页
Fujian Journal of Agricultural Sciences
基金
福州市科技计划项目(2019-N-1)
关键词
苦瓜
耐热性
主基因+多基因
遗传分析
Bitter gourd
heat tolerance
major gene + polygene
genetic analysis
