摘要
作者在上海市血液中心进修期间接收1例抗cE抗体引起迟发性溶血性输血反应患者标本,患者既往有多次输血史,对患者标本进行ABO和Rh血型鉴定、直接抗人球蛋白试验、不规则抗体筛查和抗体特异性鉴定;采用酸放散试验鉴定红细胞表面致敏抗体;采用毛细管离心技术分离患者自身和输入的红细胞。经检测患者的Rh血型为CCee,直接抗人球蛋白试验呈阳性,血清及放散液中检出抗cE抗体,远心端细胞c抗原和E抗原阳性。表明该患者体内存在抗cE抗体,因输入cE抗原阳性的红细胞引起了迟发性输血反应。
Shanghai Blood Center received a sample of patient with delayed hemolytic transfusion reaction caused by anti cE antibody.ABO and Rh blood group identification,direct anti human globulin test,irregular antibody screening and antibody specificity identification were carried out on the sample.The sensitized antibody on the surface of red blood cells was detected by acid dispersion,and the patients own and imported red blood cells were separated by capillary centrifugation.The patient had a history of multiple blood transfusions,the Rh blood group was CCee,the direct anti human globulin test was positive,anti cE antibody was detected in serum and exudate,and c antigen and E antigen were positive in distal end cells.There was anti cE antibody in this patient,and the delayed transfusion reaction was caused by inputting cE antigen positive red blood cells.
作者
张勤
郑皆炜
王中英
向东
Zhang Qin;Zheng Jiewei;Wang Zhongying;Xiang Dong(Clinical Laboratory Medicine Center,Zhengzhou Yihe Hospital Affiliated to Henan University,Zhengzhou 450018,Henan,China;Shanghai Blood Center,Shanghai 20005,China)
出处
《实用检验医师杂志》
2019年第4期244-246,共3页
Chinese Journal of Clinical Pathologist
