摘要
染料废水中存在着大量的亚甲基蓝污染物,本文以具有良好生物相容性的海藻酸铜(CA)为载体,采用包埋的形式制备漆酶和生物炭固定化海藻酸铜球(Lac-BCA),对亚甲基蓝染料进行去除研究,并探讨酶的加载特性与稳定性。实验结果表明:与游离漆酶相比,固定在CA上的漆酶在pH(3~7)和温度(10~80℃)工作范围内,具有更强的稳定性,其在4℃的条件下储存15 d,仍具有79.4%的活性;Lac-BCA对15 mg/L的亚甲基蓝在8 h的去除率达到了100.0%,且在重复利用5次后,该复合材料对亚甲基蓝的去除率稳定保持在93.4%;另外,由于高吸附性的生物炭以及漆酶的加入,相比于CA,Lac-BCA对15 mg/L的亚甲基蓝溶液去除率从46%提升至100%。
In order to remove a large amount of methylene blue in water,copper alginate(CA),a good biocompatibility material,was used as the carrier to prepare laccase and biochar encapsulated copper alginate spheres(Lac-BCA)to remove methylene blue.The loading characteristics and stability of the laccase were also investigated.The experimental results showed that the Lac-BCA had a wider operating range of pH(3-7)and temperature(10-80℃)compared with the free laccase.The relative activity of Lac-BCA could still be 79.4%when stored at 4℃for 15 d.The Lac-BCA could remove 100.0%methylene blue(15 mg/L)after 8 h,and 93.4%methylene blue could still be removed after five times of reuse.Moreover,due to the addition of highly adsorbent biochar,the removal efficiency of Lac-BCA on pollutants was increased from 46%to 100%compared with CA.
作者
朴云仙
刘再冉
张彧
李珂澜
Piao Yunxian;Liu Zairan;Zhang Yu;Li Kelan(College of New Energy and Environment,Jilin University/Key Laboratory of Groundwater Resources andEnvironment(Jilin University),Ministry of Education,Changchun 130021,China)
出处
《吉林大学学报（地球科学版）》
CAS
CSCD
北大核心
2022年第6期2014-2020,共7页
Journal of Jilin University：Earth Science Edition
基金
国家自然科学基金项目(51809111)
关键词
生物炭
亚甲基蓝
包埋
漆酶
biochar
methylene blue
encapsulating
laccase
