摘要
以八棱海棠(Malus robusta)为试材,采用盆栽试验,设置5个锰(Mn)处理水平(0、1、10、100、200 mg·kg^(-1)),分别标记为CK、Mn1、Mn10、Mn100、Mn200,研究了外源锰应用对海棠锰亚细胞分布、矿物质元素、多胺含量、多胺代谢酶及其相关酶基因表达的影响,以期为海棠对Mn的解毒机制提供参考依据。结果表明:与CK相比,随着外源Mn添加量的提高,细胞壁和细胞器的Mn比例逐渐增加,细胞质的Mn比例逐渐降低;同时高水平的Mn处理显著降低了植株Mg、Na、K和Ca含量,增加了Zn含量。基于高效液相色谱法(HPLC)表明,除不可溶性共价结合态多胺(PIS-PAs)中的精胺(Spm)含量显著增加外,其它多胺组分的腐胺(Put)、亚精胺(Spd)和Spm含量均呈递减趋势,这与多胺代谢酶活性(ADC、ODC、DAO、PAO)及相关多胺代谢酶基因(PavADC、PsaODC、ParDAO、PavPAO 2)的调控有关。综上,海棠根系Mn亚细胞分布及植株养分稳态受外源锰施用的影响,多胺含量、多胺代谢酶及其相关基因参与海棠植物对锰胁迫的解毒过程。
Taking Malus robusta as the test material,a pot experiment was used to set five Mn treatment levels(0,1,10,100,200 mg·kg^(-1)),which were labelled as CK,Mn1,Mn10,Mn100,and Mn200,respectively.The effects of exogenous Mn application on Mn subcellular distribution,mineral elements,polyamine content,polyamine metabolizing enzymes and their related enzyme gene expression in Malus robusta were investigated,in order to provide reference for the detoxification mechanism of Mn in Malus robusta.The results showed that,compared with CK,the proportion of Mn in the cell wall and organelles gradually increased,and the proportion of Mn in the cytoplasm gradually decreased as the addition of exogenous Mn increased.Meanwhile,the high levels of Mn significantly reduced the Mg,Na,K and Ca contents of the plants and increased the Zn.Furthermore,the HPLC results showed that,except for spermine(Spm),which was significantly increased in insoluble covalently bound polyamines(PIS-PAs),the putrescine(Put),spermidine(Spd)and and Spm contents of other polyamine fractions showed a decreasing trend,which was both related to the activity of polyamine metabolizing enzymes(ADC,ODC,DAO,PAO)and polyamine metabolizing enzyme genes(PavADC,PsaODC,ParDAO,PavPAO 2).In conclusion,the Mn subcellular distribution and plant nutrient homeostasis of Malus robusta roots were influenced by exogenous Mn application,and the polyamine content,polyamine metabolizing enzymes and their related genes were involved in the detoxification process of Malus robusta under Mn stress.
作者
朱晓强
吴向明
ZHU Xiaoqiang;WU Xiangming(School of Jiangsu Urban and Rural Construction College,Changzhou,Jiangsu 213147)
出处
《北方园艺》
CAS
北大核心
2022年第24期33-41,共9页
Northern Horticulture
基金
江苏高校“青蓝工程”优秀教学团队培养对象资助项目(苏教师函[2021]11号)
关键词
锰
海棠
亚细胞分布
养分元素
多胺
manganese
Malus robusta
subcellular distribution
mineral element
polyamine
