摘要
铜绿假单胞菌(Pseudomonas aeruginosa)、荧光假单胞菌(Pseudomonas fluorescens)及恶臭假单胞菌(Pseudomonas putida)是重要的致病菌与腐败菌,极易形成生物被膜,对人类健康和食品质量安全造成了严重的危害。Pel胞外多糖是假单胞菌(Pseudomonas)生物被膜的重要组成部分,通过相应的Pel系统进行合成和转运。运用生物信息学分析技术对Pel系统相关蛋白进行分析,有助于更好地理解假单胞菌生物被膜的形成机制。本研究以3种常见假单胞菌为研究对象,通过生物信息学分析技术,结合ExPASy在线工具、SignalP 4.0 Server、TMHMM-2.0、Phyre2等软件,分析了3种假单胞菌Pel胞外多糖转运系统相关蛋白PelA-G的理化性质、信号肽、跨膜区域以及三级结构和功能。结果表明,除PelE和PelG蛋白的理论等电点存在差异外,3种假单胞菌Pel系统的理化性质基本相同。荧光假单胞菌PelA蛋白不含跨膜区,铜绿假单胞菌PelE蛋白比另外两种菌多1段跨膜区,其他蛋白的跨膜区不存在明显差异。同源建模结果表明,3种菌PelA-G蛋白的折叠方式相同,三维结构能够高度重合,推测它们在Pel胞外多糖转运过程中发挥着相同的功能。本研究从生物信息学的角度揭示了3种假单胞菌Pel系统结构和功能上的共性,为该系统转运机制的进一步研究提供重要的数据基础。
Pseudomonas aeruginosa,Pseudomonas fluorescens,and Pseudomonas putida are important pathogenic and spoilage bacteria that can easily form biofilms causing serious harm to human health and food safety.Pel is an important component in the Pseudomonas biofilm and translocation of it is proposed to occur via Pel exopolysaccharide transport system.Analyzing proteins by bioinformatics in the Pel system contribute to understand the formation mechanism of Pseudomonas biofilm betterly.In the study,we analyzed the physicochemical properties,signal peptides,transmembrane region and tertiary structure of PelA-G proteins of the Pel exopolysaccharides transport system in three Pseudomonas species by ExPASy,SignalP 4.0 Server,TMHMM-2.0 and Phyre2.The results showed that the physicochemical properties of PelA-G proteins were almost the same in three Pseudomonas Pel systems,except the theoretical isoelectric points of PelE and PelG proteins.P.aeruginosa PelA protein does not contain a transmembrane region,and its PelE protein has one more transmembrane region compared to the other two strains.Three strains had the same folding pattern and the three-dimensional structure could be highly overlapping by homologous modeling,suggesting that they played the same function in the transport process of Pel exopolysaccharides.This study revealed the similarities in structure and function of three Pseudomonas Pel systems by bioinformatics and provided an important data basis for the further study on this transport system.
作者
吴倩
张昭寰
黄振华
刘静
潘迎捷
赵勇
Wu Qian;Zhang Zhaohuan;Huang Zhenhua;Liu Jing;Pan Yingjie;Zhao Yong(College of Food Science and Technology,Shanghai Ocean University,Shanghai,201306;College of Fisheries and Life Sciences,Shanghai Ocean University,Shanghai,201306;Laboratory of Quality&Safety Risk Assessment for Aquatic Product on Storage and Preservation(Shanghai),Ministry of Agriculture and Rural Affairs,Shanghai,201306;Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation,Shanghai 201306)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第9期3115-3123,共9页
Genomics and Applied Biology
基金
国家自然科学基金(32001800)
国家博士后创新人才支持计划(BX20190194)共同资助
