摘要
为系统研究竹根七根际土及内生细菌菌群结构信息,本研究利用Illumina MiSeq平台,采用高通量测序方法对野生竹根七根际土和内生细菌进行序列测定及生物信息学分析。结果表明,从竹根七根际土、根和叶共获得分类单元(OTUs)1148个,其中14个OUT为三者共有,竹根七根际土、根及叶各自特有的OTUs分别为337个、171个和308个,根际土的细菌多样性高于竹根七内生细菌的多样性。根际土细菌主要分布在变形菌门(Proteobacteria,47.5%)和放线菌门(Actinobacteria,20.6%),内生细菌主要分布在变形菌门(Proteobacteria,84.3%)。热度图分析表明,竹根七根际土及内生的细菌在属分类水平呈现显著差异。代谢预测分析显示,竹根七根际土中聚酮类化合物和萜类化合物代谢(Metabolism of Terpenoids and Polyketides)功能的细菌菌群丰度高于植物内生组织的。本研究可为竹根七根际及内生环境菌群功能的阐明和特定微生物的挖掘提供重要参考。
To study the endophytic and rhizosphere bacterial community characteristics of Tupistra chinensis Baker,based on Illumina Mi Seq high-throughput sequencing technology,the endophytic and rhizosphere bacteria from Tupistra chinensis Baker were sequenced and the biological information was analyzed in this study.A total of1148 OTUs were obtained,of which 14 were in common,337,171 and 308 OTUs were from the rhizosphere soils,root and leave respectively.The bacterial community diversity of rhizosphere soil sample was higher than that of the plant tissue sample.The dominate phylum of rhizosphere soils was Proteobacteria(47.5%)and Actinobacteria(20.6%)while the plant tissues were Proteobacteria(84.3%).The results of heat map analysis showed that there were significant differences between rhizosphere and endophytic bacteria community structure at genus level.The number of species with the metabolic function of terpenoids and polyketides of rhizosphere soil were higher than that of plant tissue sample.This study will provide an important reference for clarifying the functions of these bacteria and excavating specific microorganism from Tupistra chinensis Baker.
作者
安超
马赛箭
薛文娇
刘晨
丁浩
An Chao;Ma Saijian;Xue Wenjiao;Liu Chen;Ding Hao(Microbiology Institute of Shanxi,Xi'an,710043;Engineering Center of Qinling Mountains Natural Products,Shanxi Academy of Sciences,Xi'an,710043)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2021年第5期2076-2082,共7页
Genomics and Applied Biology
基金
国家自然科学基金项目(21576160)
陕西省科学院科技计划项目(2018nk-01,2018k-09)
陕西省科技厅一般项目—农业领域(2017NY-191,2018NY-152,2019NY-209)
榆林市科技计划项目(2019-186)共同资助
关键词
竹根七
内生菌
根际微生物
多样性
高通量测序
Tupistra chinensis Baker
Endophytes
Rhizosphere microorganisms
Diversity
High-throughput sequencing
