摘要
为研究白芍(RPA)的水提物对草酸钙(CaOx)结石形成的影响以及潜在的作用机理,本研究使用体外测定来确定白芍提取物是否阻止草酸钙的形成或促进草酸钙的溶解,并研究提取物在体内作为大鼠实验诱导的草酸钙肾结石病的预防和治疗剂的功效;在体内实验结束时,评估肾脏组织和尿液中的各种生化,分子和组织学参数。结果显示,与对照处理相比,在用80 mg/mL RPA提取物处理后,在体外观察到形成的晶体显着溶解并抑制了晶体形成。在预防方案中,与对照相比,白芍水提取物显着降低了尿液和肾脏的草酸水平,并增加了尿钙和柠檬酸水平;此外,白芍水提取物预防方案可显着降低骨桥蛋白的表达、肾结晶和病理变化;而在治疗方案的大鼠中未观察到这些变化。结果表明白芍水提取物是防止草酸钙肾结石形成的有用药物。
To examine the effect of an aqueous extract of Radix Paeoniae Alba(RPA)on the formation of calcium oxalate(CaOx)stones and the potential mechanism of action.In vitro assays was used to determine whether the RPA extract prevents the formation of CaOx or promotes CaOx dissolution.The study also investigated the efficacy of the extract in vivo as a preventive and therapeutic agent for experimentally induced CaOx nephrolithiasis in rats.Various biochemical,molecular,and histological parameters were assessed in kidney tissue and urine at the end of the in vivo experiment.Significant dissolution of formed crystals and inhibition of crystal formation were observed in vitro after treatment with 80 mg/mL of the RPA extract compared with a control treatment.In preventive protocols,the RPA extract significantly reduced urinary and renal oxalate levels and increased urinary calcium and citrate levels compared to the control.In addition,the RPA preventive protocol significantly decreased osteopontin expression,renal crystallization,and pathological changes compared to the control.While these changes were not observed in rats treated with the treatment regimen.The results showed that the extract of Paeoniae is a useful drug to prevent CaOx kidney stone formation.
作者
苟晓燕
游芳凝
王晓磊
李琦晖
Gou Xiaoyan;You Fanning;Wang Xiaolei;Li Qihui(Chongqing Traditional Chinese Medicine Hospital,Chongqing,400042)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第9期4365-4371,共7页
Genomics and Applied Biology
基金
科研院所绩效激励引导专项项目(cstc2017jxjl130042)资助
关键词
白芍
大鼠
草酸钙
肾结石
Radix paeoniae alba
Rat
Calcium oxalate
Nephrolithiasis
