摘要
为了考察黑灵芝多糖提取物对力竭运动小鼠的抗疲劳作用。本研究以6周龄雄性昆明小鼠为研究对象,将50只小鼠随机分为安静组(不运动)、模型组(力竭跑台运动)、低剂量组、中剂量组和高剂量组,每组10只。低、中、高剂量组分别按照50 mg/kg·bw、100 mg/kg·bw、200 mg/kg·bw的剂量对小鼠灌胃黑灵芝多糖提取液4周。研究显示,黑灵芝多糖提取物可以剂量依赖性方式提高小鼠的跑台运动时间(p<0.05);黑灵芝多糖提取物可显著减轻小鼠骨骼肌病理改变(p<0.05);黑灵芝多糖提取物可显著提高小鼠的肝糖原和肌糖原含量(p<0.05);黑灵芝多糖提取物可提高小鼠骨骼肌组织中SOD、CAT和GSH-Px含量并降低MDA水平(p<0.05);黑灵芝多糖提取物可显著上调小鼠肝组织中ORM1和CCR5的m RNA和蛋白水平。本研究表明黑灵芝多糖提取物可有效提高运动小鼠的抗疲劳能力。黑灵芝多糖提取物的抗疲劳作用与预防骨骼肌损伤、增加糖原储备、提高抗氧化活能力并降低氧化应激损伤有关。此外,黑灵芝多糖提取物可通过激活ORM1-CCR5信号来发挥抗疲劳作用。
To investigate the anti-fatigue effect of polysaccharide extract from Ganoderma atrum on exhausted mice.In this study,6-week-old male Kunming mice were used as research objects.Fifty mice were randomly divided into a quiet group(without exercise),a model group(exhausted treadmill exercise),a low-dose group,a medium-dose group,and a high-dose group(n=10).Low,medium,and high dose groups were administered with G.atrum polysaccharide extract at a dose of 50 mg/kg·bw,100 mg/kg·bw,200 mg/kg·bw to mice for 4 weeks.Studies showed that G.atrum polysaccharide extract increased the running time of mice in a dose-dependent manner(p<0.05).G.atrum polysaccharide extract significantly reduced pathological changes in skeletal muscle of mice(p<0.05).G.atrum polysaccharide extract significantly increased liver glycogen and muscle glycogen content in mice(p<0.05).G.atrum polysaccharide extract increased the content of SOD,CAT and GSH-Px in skeletal muscle tissue of mice and reduced the level of MDA(p<0.05).G.atrum polysaccharide extract significantly increased the mRNA and protein levels of ORM1 and CCR5 in mouse liver tissue.This study indicates that the polysaccharide extract of G.atrum can effectively improve the anti-fatigue ability of exercised mice.The anti-fatigue effect of polysaccharide extract from G.atrum is related to preventing skeletal muscle damage,increasing glycogen reserve,improving antioxidant activity and reducing oxidative stress damage.In addition,G.atrum polysaccharide extract can exert anti-fatigue effect by activating the ORM1-CCR5 signal.
作者
万利
Wan Li(Institute of Physical Education,Hubei Minzu University,Enshi,445000)
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2020年第9期4339-4344,共6页
Genomics and Applied Biology
基金
湖北省教育厅科技研究项目(D20201901)
湖北省教育科学规划课题2018年度课题(2018GB032)共同资助
