摘要
基于电阻抗的细胞分析是一种无标记、非侵入性技术,已广泛用于分析和识别各种应用中的细胞特征,如组织培养、细胞生长及活力检测等。微流体(也称为“芯片实验室”)技术能够将涉及样品制备、操作和基于阻抗检测的分析过程集成到单片芯片上,最大限度地减少系统尺寸、重量。本文搭建了一种简便的微流控电阻抗检测系统,设计了微流控阻抗芯片,采用自制的锁相放大器放大感应信号,并在MATLAB上利用自研算法处理,提取出特征参数并进行分析。该芯片可根据电学差异对体积相近的不同微粒进行区分,具有成本低、易集成、非标记等优势,未来可进一步用于现场细胞即时检测。
Cell analysis based on electrical impedance is a label-free and non-invasive technique,which has been widely used to analyze and identify cell characteristics in various applications,such as tissue culture,cell growth and viability detection.Microfluidic(also known as"Lab on a Chip")technology can integrate analytical processes involving sample preparation,operation and impedance-based detection into a single chip,minimizing the size and weight of the system.In this paper,a simple microfluidic impedance detection system is built,and a microfluidic impedance chip is designed.The induction signal is amplified by a self-made lock-in amplifier and processed by self-developed algorithm on MATLAB,and the characteristic parameters are extracted and analyzed.Different particles of similar size can be distinguished according to electrical differences,which has the advantages of low cost,easy integration and non-labeling,and can be further used for on-site cell real-time detection in the future.
作者
周扬
方维翰
刘婷
贾春平
赵建龙
ZHOU Yang;FANG Wei-han;LIU Ting;JIA Chun-ping;ZHAO Jian-long(Shanghai Institute of Microsystems and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China;Shanghai Pinghe School,Shanghai 201203,China)
出处
《功能材料与器件学报》
CAS
2022年第5期458-462,共5页
Journal of Functional Materials and Devices
基金
中国科学院关键技术研发团队项目(GJJSTD20210006)
关键词
微流控
流式阻抗分析
锁相放大器
聚苯乙烯微球
Microfluidics
Flow Impedance Analysis
Lock-in Amplifier
Polystyrene Microspheres
