摘要
目的研究胰高血糖素肽-1受体激动药(Exendin-4)对血管紧张素Ⅱ(AngⅡ)诱导的心肌成纤维化的作用及其机制。方法用Ⅱ型胶原酶消化心脏组织并结合差速贴壁法分离纯化的心肌成纤维细胞(CFb);将CFb分为7组:空白组(无任何处理),模型组(AngⅡ100 nmol·L^-1),阳性对照组(洛沙坦10μmol·L^-1),抑制剂组(U012630μmol·L^-1)和低、中、高3个剂量实验组(Exendin-41,5,10 nmol·L^-1)。四唑盐法检测细胞增殖率;以免疫印迹试验测定细胞CollagenⅠ、磷酸化ERK1/2和磷酸化AKT蛋白的相对表达水平(灰度值)。结果空白组、模型组和低、中、高3个剂量实验组的细胞增殖率分别为(7.31±2.18)%,(30.31±3.88)%,(28.93±2.18)%,(20.17±2.19)%和(17.85±2.36)%;模型组与空白组相比、或者低、中、高3个剂量实验组与模型组相比,差异均有统计学意义(均P<0.01)。空白组、模型组、高剂量实验组和阳性对照组的Collagen I相对表达量分别为0.96±0.08,1.30±0.07,1.01±0.07和0.99±0.05;这4组磷酸化ERK的相对表达量分别为0.52±0.07,1.15±0.26,0.69±0.28和0.64±0.06;这4组的磷酸化AKT的相对表达量分别是0.68±0.18,1.03±0.23,0.70±0.19和0.69±0.18。模型组与空白组相比,差异均有统计学意义(P<0.05或P<0.01);高剂量实验组与模型组相比,差异均有统计学意义(P<0.05或P<0.01)。结论Exendin-4可预防Ang II诱导的心肌纤维化,其作用机制可能通过抑制CFb的增殖、下调ERK和AKT蛋白磷酸化水平,从而减少Collagen I合成来发挥作用的。
Objective To study the preventive effect and possible mechanism of glucagon peptide-1 receptor agonist(Exendin-4)on angiotensinⅡ(AngⅡ)induced myocardial fibrosis.Methods Cardiac tissue was digested with typeⅡcollagenase and separated into purified cardiac fibroblasts(CFb)by differential adherence methods.Cells were divided into seven groups:blank group(without any treatment),model group(AngⅡ100 nmol·L^-1),positive control group(losartan 10μmol·L^-1),inhibitor group(U012630μmol·L^-1),and low,middle,high experimental groups(Exendin-41,5,10 nmol·L^-1).The cell proliferation rate was detected by tetrazolium salt method.The relative expression levels(grey value)of Collagen I,phosphorylated ERK1/2and phosphorylated AKT protein were determined by Western blot.Results The cell proliferation rate in blank group,model group and low,middle,high experimental groups were(7.31±2.18)%,(30.31±3.88)%,(28.93±2.18)%,(20.17±2.19)%,(17.85±2.36)%,respectively;comparing between model group and normal group,the difference was significant(P<0.01);comparing between three doses experimental groups and model group,the difference were significant(all P<0.01).The relative expression levels of Collagen I in blank group,model group,high-dose experimental group,and positive control group were 0.96±0.08,1.30±0.07,1.01±0.07,and0.99±0.05,respectively;the relative expression levels of phosphorylation ERK in the 4 groups were 0.52±0.07,1.15±0.26,0.69±0.28 and 0.64±0.06.The relative expression levels of phosphorylated AKT in the four groups were 0.68±0.18,1.03±0.23,0.70±0.19 and 0.69±0.18,respectively.Comparing between model group and normal group,the difference of the factors were significant(P<0.05 or P<0.01);comparing between experimental group and model group,the difference of the factors were significant(P<0.05 or P<0.01).Conclusion Exendin-4can prevent AngⅡ-induced myocardial fibrosis,and its mechanism may play a role in inhibiting the proliferation of CFb,down-regulating ERK,AKT protein phosphorylation and reducing Collagen I synthesis.
作者
马楠
金红花
MA Nan;JIN Hong-hua(Department of Pharmacy,Yanbian University,Yanji 133002,Jilin Province,China;Department of Pharmacy,Yanbian University Affliated Hospital,Yanji 133002,Jilin Province,China)
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2020年第3期271-273,共3页
The Chinese Journal of Clinical Pharmacology
基金
国家自然科学基金资助项目(81860077).
