摘要
建立一种高效快捷的化妆品中防腐剂卡松(甲基氯异噻唑啉酮(CIT)和甲基异噻唑啉酮(MIT))含量测定的高效液相色谱检测方法。方法采用高效液相色谱法(HPLC),选用ZORBX C18色谱柱(4.6 mm×250 mm,5μm),以1.0 m L/min甲醇︰水(V︰V=70︰30)为流动相,二极管阵列检测器为检测器,外标法进行定量分析。结果CIT和MIT的线性范围分别为0.0208~0.1559 g/L和0.0104~0.0782 g/,CIT和MIT的浓度和峰面积的线性良好,相关系数均为1.0000(R2>0.999),该方法的检出限分别为6.93×10-6 g/g和0.568×10-6 g/g,定量限分别为2.31×10-5 g/g和1.89×10-6 g/g,在不同的添加水平下,该方法的回收率范围分别为86.362%~91.926%和88.126%~94.555%,相对标准偏差分别为2.2%和2.9%(n=6)。结论高效液相色谱法灵敏度高、准确,适用于化妆品中防腐剂卡松(CIT和MIT)的含量测定。
To establish an efficient solution for determination of the content of the preservative kathon(methyl chlorothiazoline(CIT)and methylisothiazoline(MIT))in cosmetics by a high performance liquid chromatography.Method:Using high performance liquid chromatography(HPLC),ZORBX C18 chromatographic column was selected(4.6 mm×250 mm,5μm),take the 1.0 m L/min methanol:water(V:V=70:30)as mobile phase,the diode array detector as a detector,the external standard method is used for quantitative analysis.Result:The linear range of the CIT and MIT respectively are 0.0208~0.1559 g/L and 0.0104~0.0782 g/L,CIT and MIT have good linear concentrations and peak area,all the correlation coefficients are 1.0000(R2>0.999),the detection limits of this method are respectively 6.93×10-6 g/g and 0.568×10-6 g/g,the quantitative limits are respectively 2.31×10-5 g/g and 1.89×10-6 g/g,at different levels of addition,the recovery range of this method is respectively 86.362%~91.926%and 88.126%~94.555%,the relative standard deviation is 2.2%and 2.9%(n=6).Conclusion:High performance liquid chromatography is high sensitivity and accuracy,it is suitable for the determination of the content of the preservative kathon(CIT and MIT)in cosmetics.
作者
陈晓霞
谭小玲
杜冠星
周立武
武升虎
Chen Xiaoxia;Tan Xiaoling;Du Guaning;Zhou Liwu;Wu Shenghu(Quality Control Center,Easycare(ZHUHAI)Technology Co.,Ltd.,Zhuhai 519055,China)
出处
《广东化工》
CAS
2020年第3期191-193,共3页
Guangdong Chemical Industry
关键词
高效液相色谱法
卡松
甲基异噻唑啉酮
甲基氯异噻唑啉酮
化妆品
含量测定
high performance liquid chromatography
Kathon
Methylisothiazolinone
Methylchloroisothiazo-lineketone
cosmetics
content determination
