摘要
Objective:To evaluate the antidiabetic and antioxidant effects of the methanol extract of Syzygium operculatum bark(MSOB)using in vivo and in vitro models.Methods:Antidiabetic activity was assessed by using alloxan induced(120 mg/kg body weight)diabetic mice.The plant extract(200 mg/kg and 400 mg/kg body weight)was administrated i.p.to diabetic mice in comparison with standard metformin hydrochloride(150 mg/kg body weight).The antioxidant activity of the extract was evaluated by using a range of in vitro assays and results were compared to standards.Results:The extract MSOB 200 mg/kg and 400 mg/kg reduced the blood glucose level 44.05%and 55.53%respectively where the standard drug metformin reduced 69.42%in dose of 150 mg/kg body weight.The extract MSOB showed significant 1,1-diphenyl-2-picrylhydrazyl scavenging activity,total phenol content,total flavonoid content and reducing power capacity compared to standards.The IC_(50)values were found 300.34μg/mL in 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay where 40.31μg/mL for ascorbic acid.The total phenol and flavonoid contents were 197.5 and 267.5 mg of gallic acid equivalent per gram,respectively.Conclusions:The present study indicates that the extract of Syzygium operculatum bark is the potential sources of natural antioxidant and possesses significant antidiabetic activities.
基金
Supported by Department of Pharmacy,International Islamic University Chittagong,Bangladesh(Grant No.Pharm-58/07-01’14).
