摘要
目的:采用HPLC法同时测定蓝花参中芦丁、 橙皮苷及槲皮素的含量,为该药材的深入研究及资源开发提供参考依据.方法:采用HPLC-DAD法测定芦丁、 橙皮苷及槲皮素含量,检测波长268 nm,乙腈(A)-0.5%乙酸水(B)溶液为流动相进行梯度洗脱.结果:芦丁:y=0.128x+0.279(r=0.9970),线性范围为3.848~48.10μg/mL;橙皮苷:y=0.101x+0.401(r=0.9965),线性范围为4.144~82.88μg/mL;槲皮素:y=0.097x+0.007(r=0.9990),线性范围为1.02~6.12μg/mL.结论:该法精密度、 准确性良好,方法简便快捷,可用于蓝花参中芦丁、 橙皮苷及槲皮素的含量测定.
Objective To provide a reference for resource development about Wahlenbergia marginata(Thunb.)A.DC.by qualitative analysis of the rutin,hesperidin and quercetin from this medicinal herb.Methods The content of rutin,hesperidin and quercetin was determined by HPLC-DAD with mobile phase of acetonitrile(A)-0.5%acetic acid(B)for gradient elution and detection wavelength of 268 nm.Result Rutin:y=0.128x+0.279(r=0.9970),linear range:3.848~48.10μg/mL;hesperidin:y=0.101x+0.401(r=0.9965),linear range:4.144~82.88μg/mL;quercetin:y=0.097x+0.007(r=0.9990),linear range:1.02~6.12μg/mL.Conclusion The method is accurate,simple and rapid,and can be used for the determination of the rutin,hesperidin and quercetin in Wahlenbergia marginata(Thunb.)A.DC.
作者
张宽
曾茂贵
罗兰
李颖
叶华
张文清
ZHANG Kuan;ZENG Maogui;LUO Lan;LI Ying;YE Hua;ZHANG Wenqing(The Second People’s Hospital of Fujian province,Fuzhou 350003,China;Fujian Health College,Fuzhou 350101,China;Fujian Provincial Governmental Hospital Fuzhou 350101,China)
出处
《中国民族民间医药》
2019年第12期42-45,共4页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
福建省自然科学基金项目(2014J01039)
关键词
蓝花参
HPLC
芦丁
橙皮苷
槲皮素
Wahlenbergia marginata(Thunb.)A.DC.
HPLC
Rutin
Hesperidin
Quercetin
