摘要
目的:建立参乌益肾片中芍药苷的含量测定方法。方法采用水浴回流提取,HPLC测定法,用C18柱,以乙腈-0.1%磷酸溶液(15:85)为流动相,检测波长:230nm。结果用甲醇水浴回流30 min的方法提取参乌益肾片中芍药苷,HPLC法测定芍药苷含量,精密度的RSD%为0.2%,重复性RSD%为0.3%,加样回收率为98.6%。结论采用回流提取HPLC法测定参乌益肾片中芍药苷方法可行。
Objective To establish and kidney in wu yi paeoniflorin content determination method.Methods The water bath reflux extraction,HPLC assay,using C18 column,acetonitrile-0.1%acetonitrile(15:85)as mobile phase,the detection wavelength:230nm.Results 30 minutes with methanol water bath reflux method to extract and kidney in wu yi paeoniflorin,the HPLC method for the determination of the content of Rutin the precision of the RSD%0.2%,repetitive RSD%0.3%,the rate of recovery was 98.6%.Conclusion Using the method of reflux extraction and HPLC determination and kidney.
作者
刘琴
陈美玲
张娟
霍艳萍
高晓艳
LIU Qin;CHEN Mei-li;ZHANG Juan;HUO Yan-ping;GAO Xiao-yan(Jiangsu Kanion Pharmaceutical Limited by Share Ltd,Lianyungang 222047,Jiangsu,China)
