摘要
目的探讨肝癌患者KPNA2表达与凝血指标变化及其临床意义。方法收集我院2012年1月~2013年8月肝癌患者30例,正常人对照40例。采用RT-PCR法从转录水平上检测KPNA2在肝癌组织及癌旁正常肝组织中的表达水平。用法国STA COMPACT CT全自动血凝分析仪检测患者及正常人对照的凝血酶原时间(PT)、活化部分凝血活酶时间(APTT)、纤维蛋白原(Fib)、凝血酶时间(TT),并与对照进行比较。结果30例肝癌患者中25例(83.33%)肝癌组织KPNA2的mRNA灰度值与癌旁正常肝组织之比>2倍。凝血指标PT、APTT、TT、FIB含量±次为:肝癌组14.0±1.3,35.8±6.1,3.76±1.31,18.9±1.7;正常对照组为12.9±0.8,34.0±4.4,3.1±0.5,16.4±1.1。经统计学处理,肝癌组织KPNA2的mRNA灰度值与癌旁正常肝组织有显著性差异(<0.01);凝血指标肝癌组与正常对照组比较:血浆PT、APTT、TT、FIB含量均存在着显著性的差异(<0.05),有统计学意义。结论 KPNA2在肝癌组织中高表达,在癌旁正常肝组织中低表达可作为肝癌诊断的指标;监测肝癌患者血浆PT、APTT、TT、FIB的含量变化,可较好地了解患者肝功能的状况,为评估患者肝脏受损程度和病情判断及治疗效果观察提供科学±据。
Objective To detect the expression of KPNA2 and coagulation indicator in hepatocellular carcinoma,find the prognostic marker of liver cancer.Methods We col ected liver cancer samples from 30 patients who lived in Suining central hospital from January 2012 to august 2013.30 para-tumor tissues and 40 healthy blood samples as control,detected the mRNA expression of KPNA2 by RT-PCR,prothrombin time(PT),activated partial thromboplastin time(APTT),fibrinogen,and thrombin time(TT)by automatic blood coagulation analyzer(STA COMPACT CT).Results The mRNA expression of KPNA2 in liver cancer is higher than para-tumor in 83.33%patients(25/30).PT,APTT,TT,FIB are 14.0±1.3,35.8±6.1,3.76±1.31,18.9±1.7 in patients group;12.9±0.8,34.0±4.4,3.1±0.5,16.4±1.1 in control group.The difference is considered statistical y significant.Conclusion KPNA2 could be the biomarker of hepatocellular carcinoma.Monitoring the coagulation indicator could improve the understand of liver function of patients,provide the scientific basement to improve therapeutic effect.
作者
李凤
蒲泽晏
刘方久
魏容
梁艳丽
陈婕
冯簏洁
LI Feng;PU Ze-yan;LIU Fang-jiu;WEI Rong;LIANG Yan-li;CHEN Jie;FENG Lu-jie(Test Department,Suining City Central Hospital,Suining 629000,Sichuan,China;Basic Laboratory,Suining City Central Hospital,Suining 629000,Sichuan,China;不详)
关键词
肝细胞癌
KPNA2
凝血指标
Hepatocellular carcinoma
KPNA2
Coagulation indicator
