摘要
目的:比较分析三种梅毒检测方法,提高梅毒临床诊断符合率。方法收集2012年10月~2013年10月门诊及住院患者共8178例,经梅毒酶联免疫吸附试验(TP-ELISA)筛选出200例血清标本分别用梅毒螺旋体明胶颗粒凝集试验(TPPA)和甲苯胺红不加热血清试验(TRUST)法进行检测,以TPPA检测结果作为确诊。结果以TPPA为标准可得出TP-ELISA的敏感性较高;而TRUST的敏感性较低,差异有统计学意义(P<0.05)。结论2种方法的特异性均较高,无明显差异(P>0.05)。 TP-ELISA适合大规模筛查和临床的诊断,TRUST敏感性较低,不适合于大批量筛查,为提高梅毒的检出率和准确性,建议用TPPA复检和确诊,3种检测方法结合应用能更有效提高梅毒临床诊断符合率。
Objective To compare three methods for detection of syphilis,improve the clinical diagnosis of syphilis.Methods A total of 8178 cases of syphilis including outpatients and inpatients were col ected from 2012 October to 2013 October,among which 200 serum samples selected by TP-ELISA were detected by TPPA and TRUST methods,the TPPA results as confirmation.Results Can be obtained with the standard of TPPA that the sensitivity of TP-ELISA was higher and TRUST was lower,the dif erence was statistical y significant(P<0.05).Conclusion Specificity of the two methods was high,no significant dif erence(P>0.05).TP-ELISA method is suitable for large-scale clinical screening and diagnosis,the TRUST method is not suitable for mass screening because of the lower sensitivity,in order to improve the detection rate and accuracy of syphilis,we advice examing and confirming by TPPA.Three kinds of detection methods combined application can more ef ectively improve the rates of clinical diagnosis.
作者
陈磊
孙秀凤
CHEN Lei;SUN Xiu-feng(The Fourth Central Hospital of Tianjin City,Tianjin 300140,China)
关键词
梅毒
酶联免疫吸附试验
梅毒螺旋体明胶颗粒凝集试验
甲苯胺红不加热血清试验
Syphilis
Enzyme-linked immunosorbent assay
Treponema pallidum gelatin particle agglutination test for syphilis
Methylaminered unheated serum test
