摘要
目的:探讨我院血培养标本的病原菌分布特征和耐药性情况,指导临床合理使用抗生素。方法采用BD BACTEC 9120型全自动血培养仪进行细菌培养,经VITEK2 Compact全自动微生物分析系统进行细菌鉴定及药敏试验,应用WHONET5.1软件进行数据分析。结果2242例血培养标本共检出阳性菌株118株,阳性率为5.2%。其中革兰氏阳性菌53株、革兰氏阴性菌65株,分别占总数的44.9%和55.1%,革兰阳性菌以凝固酶阴性葡萄球菌为主,对万古霉素、利奈唑胺、替加环素、奎奴普汀/达福普汀较敏感,革兰氏阴性菌以大肠埃希菌为主,对厄他培南、亚胺培南、头孢替坦、阿米卡星、哌拉西林/他唑巴坦较敏感。结论我院血培养分离的病原菌以革兰阴性菌为主,有较高的耐药率,提醒需要加强对血液感染病原菌的监控,督促临床医生合理使用抗生素。
Objective To investigate the distribution and drug resistance of pathogenic bacteria in blood culture specimens,and guide rational use of antibiotics in clinic.Methods Blood samples were cultivated by the BD BACTEC 9120 type automatic blood culture instrument,pathogenic bacteria were identified and drug sensitive tests were tested by VITEK2 Compact Automatic Analyzer for Microbes,and corresponding results were analyzed with WHONET5.5 software.Results There were 118 strains were isolated from 2242 blood samples with the positive rate of 5.2%,including 53 gram-positive strains and 65 gram-negative strains,accounting for 44.9%and 55.1%of the total,respectively.Gram-positive bacteria were predominantly coagulase negative staphylococcus,was sensitive to vancomycin,macrodantin,kuiNuleptin/dafoeleptin.Gram-negative bacteria were predominantly escherichia coli,was sensitive to cefotetan,etapenem,imipenem,amikacin,piperacil in/tazobactam.Conclusion Blood culture pathogenic bacteria isolated were predominantly gram-negative bacteria and antibiotic resistance rate is high,It reminded need to strengthen the monitoring of blood infection pathogens and supervise the clinical doctor to use of antibiotics rational y.
作者
董政
童明华
梁日初
DONG Zheng;TONG Ming-hua;LIANG Ri-chu(Hunan Wangwang Hospital,Department of Clinical Laboratory,Changsha,Hunan,410016,China)
关键词
血培养
病原菌
耐药性
Blood culture
Pathogenic bacteria
Drug resistance
