摘要
Objective: To study the correlation of FUT3 gene polymorphism with immune response and inflammatory response in patients with ulcerative colitis (UC). Methods: Patients with UC and patients with irritable bowel syndrome who accepted endoscopic biopsy in the First Affiliated Hospital of Jiamusi University between May 2014 and April 2017 were selected as UC group and control group respectively, and the FUT3 gene polymorphism, immune transcription factor and inflammation molecule expression as well as immune cell contents in intestinal mucosa were determined. Results: FUT3 gene rs28362459 and rs3894326 locus genotype constituent ratio in intestinal mucosal tissue of UC group were not significantly different from those of control group whereas rs3745635 locus GA+AA genotype constituent ratio was significantly higher than that of control group;CD4+CD29+ and CD4+IL17A+ cell contents as well as HSF-2, NF-kB, Bax, TNF-α and IL-1β mRNA expression in mucosal tissue of UC group were significantly higher than those of control group whereas CD4+CD25+Foxp3+ cell content as well as SOCS2 and SOCS3 mRNA expression was significantly lower than those of control group;CD4+CD29+ and CD4+IL17A+ cell contents as well as HSF-2, NF-kB, Bax, TNF-α and IL-1β mRNA expression in UC group of mucosal tissue with FUT2 rs3745635 locus GA+AA genotype were significantly higher than those of UC group of mucosal tissue with GG genotype whereas CD4+CD25+Foxp3+ cell content as well as SOCS2 and SOCS3 mRNA expression was significantly lower than those in UC group of mucosal tissue with GG genotype. Conclusion: The FUT3 gene rs3745635 locus polymorphism in intestinal mucosa of UC is closely related to immune response and inflammatory response disorders.
基金
Project of Heilongjiang Provincial Natural Science Foundation(No.:H201367).Scientific Research Project of Health Department of Heilongjiang Province(:2013245).
