摘要
目的评价碘元素自动检测仪检测尿碘方法的可行性。方法应用碘元素自动检测仪测定尿碘含量,在50~1000μg/L标准曲线范围内,开展标准曲线线性关系、精密度和准确度(尿碘标准物质GBW09108x、GBW09110c、GBW09111b、GBW09112b测定及加标回收实验)等方法学评价;并与国家碘缺乏病参照实验室(NRL)推荐的砷铈催化分光光度法进行方法比对实验。结果碘元素自动检测仪在50~1000μg/L标准曲线范围内有良好的线性关系,│r│均>0.9990;低、中、高3种碘浓度尿样测定碘含量的变异系数分别为2.4%、0.8%、1.1%;低、中、高3种碘浓度尿样加标回收率范围为93.0%~104.1%,总平均回收率为98.9%。尿碘标准物质GBW09108x、GBW09110c、GBW09111b、GBW09112b的测定结果均在给定标准值±不确定度范围内[(80.8±9.0)、(227.0±15.0)、(525.0±17.0)、(794.0±28.0)μg/L]。应用碘元素自动检测仪与NRL推荐标准方法测定结果比较,差异无统计学意义(t=-1.94,P=0.062)。结论碘元素自动检测仪测定尿碘自动化程度高、环境适应性强、检测效率优,方法学特性实验结果良好,可以用于尿碘样品检测。
Objective To evaluate the feasibility of using an automatic iodine detector to detect urinary iodine.Methods Using an automatic iodine detector to determine urinary iodine,within the range of the 50-1000μg/L standard curve,methodological evaluations on the linear relationship of the standard curve,precision,and accuracy(determination of urinary iodine standard substances GBW09108x,GBW09110c,GBW09111b,GBW09112b,and spiked recovery experiments)were conducted.Method comparison experiments with the arsenic cerium catalytic spectrophotometry recommended by the National Reference Laboratory for Iodine Deficiency Disorders(NRL)was conducted.Results The method has a good linear relationship within the range of 50-1000μg/L standard curve,│r│>0.9990.The coefficient variations for measuring iodine levels in urine samples with low,medium,and high iodine concentrations were 2.4%,0.8%,and 1.1%,respectively.The recovery rates of urine samples with low,medium,and high iodine concentrations ranged from 93.0%to 104.1%,with a total average recovery rate of 98.9%.The determination results of standard substances GBW09108x,GBW09110c,GBW09111b,and GBW09112b were all within the given standard value±uncertainty range[(80.8±9.0),(227.0±15.0),(525.0±17.0),(794.0±28.0)μg/L].There was no statistically significant difference between the results obtained by using the iodine automatic detector and the NRL recommended standard method(t=-1.94,P=0.062).Conclusions The iodine automatic detector has a high degree of automation,strong environmental adaptability,and excellent detection efficiency in determining urinary iodine.The experimental results of methodological characteristics are good and can be used for the detection of urinary iodine samples.
作者
李科
王慧
杨志羡
李艳红
Li Ke;Wang Hui;Yang Zhixian;Li Yanhong(Institute for Endemic Disease Control,Inner Mongolia Autonomous Regional Center for Disease Control and Prevention(Inner Mongolia Autonomous Regional Academy of Preventive Medicine),Hohhot 010080,China)
出处
《中华地方病学杂志》
CAS
北大核心
2024年第3期239-242,共4页
Chinese Journal of Endemiology
关键词
尿
碘
碘元素自动检测仪
Urine
Iodine
Automatic iodine detector
