摘要
目的采用液相色谱质谱(LC-MS)的代谢组学分析方法,研究急性白血病患者的血清代谢组学特征,寻找与急性白血病相关的潜在标志物,初步探讨其涉及的相关代谢通路。方法采用基于LC-MS的代谢物分析方法,分析63名急性髓系白血病和急性淋巴细胞白血病的患者,以及59名健康供者血清中的小分子代谢物。用多变量数据分析及t检验筛选差异代谢物,并通过MetaboAnalyst 4.0分析平台探讨差异代谢物相关的代谢通路。结果应用LC-MS代谢组学分析方法可区分急性白血病患者和健康供者。结合变量权重重要性值及t检验筛选到29种差异代谢物,相对于健康供者,急性白血病患者代谢异常,主要涉及亚油酸代谢和苯丙氨酸、酪氨酸、色氨酸以及精氨酸等氨基酸生物合成代谢通路。结论LC-MS代谢组学可以鉴定急性白血病患者的血清内源性差异代谢物,筛选出差异代谢物对急性白血病患者的临床诊断和预测其发病风险具有潜在的临床应用价值。
Objective To investigate whether acute leukemia patients have unique serum metabolomics characteristics by using liquid chromatography-mass spectrometry(LC-MS),to search for potential markers related to acute leukemia,and to preliminarily explore the related metabolic pathways involved.Methods A metabolite analysis based on LC-MS was perform to analyze the small molecule metabolites in the serum of 63 acute leukemia patients and 59 healthy donors.Multivariate analysis and t-test were used to screen the differential metabolites,and the metabolic pathways related to differential metabolites were explored through the MetaboAnalyst 4.0 analysis platform.Results LC-MS-based metabolomics analysis can be used to distinguish acute leukemia patients from healthy donors.Based on VIP value and t-test,29 different metabolites were screened,and metabolic abnormalities were found in acute leukemia patients compared with healthy donors,mainly involving linoleic acid metabolism and phenylalanine,tyrosine,tryptophan,and arginine biosynthesis pathways.Conclusion LC-MS-based metabolomics can identify unique endogenous differential metabolites in the serum of acute leukemia patients,and the screened differential metabolites have potential for the clinical diagnosis and prediction of the risk of acute leukemia.
作者
王磊
李梦
孙文利
刘瑞
王红春
卢光泽
赵颖
郭进艳
刘红星
Wang Lei;Li Meng;Sun Wenli;Liu Rui;Wang Hongchun;Lu Guangze;Zhao Ying;Guo Jinyan;Liu Hongxing(Department of Laboratory Medicine,Hebei Yanda Ludaopei Hospital,Langfang 065201,China;Department of Laboratory Medicine,Beijing Ludaopei Hospital,Beijing 100176,China)
出处
《中华临床医师杂志（电子版）》
CAS
北大核心
2023年第8期850-857,共8页
Chinese Journal of Clinicians(Electronic Edition)
基金
河北省医学研究课题(20210319)
关键词
代谢组
白血病
液相色谱质谱
代谢通路
Metabolomic
Leukemia
Liquid chromatography-mass spectrometry(LC-MS)
Metabolism pathway
