摘要
目的评价自制改良哥伦比亚巧克力培养基（ＩＣＣＡ）对流感嗜血杆菌（Ｈｉｎ）的分离培养效果。方法将ＨｉｎＡＴＣＣ４２９４７接种于ＩＣＣＡ和其他４种培养基上，计算和比较５种培养基Ｈｉｎ的平均生长指数；用ＩＣＣＡ对４４８份痰标本进行检测，比较其与血琼脂加金葡菌划线（ＢＡＳＳ）方法的Ｈｉｎ检出率。结果与其他４种培养基比较，ＩＣＣＡ的Ｈｉｎ平均生长指数为１７．７９，痰标本中的Ｈｉｎ的检出率为２０．１％，ＩＣＣＡ明显高于ＢＡＳＳ（检出率１２％），经χ２检验Ｐ＜００１。结论ＩＣＣＡ是一种较好的Ｈｉｎ选择性培养基。
Objective To evaluate isolating efficacy of an improved chocolated Columbia Agar (ICCA) for Haemophilus influenzae.Methods We evaluated ICCA with other four selective media for the ability of the growth of Hin (ATCC49247) by using a growth index (GI) that combines colony sites and the dilution factor, and we also used this ICCA medium to isolate Hin from 448 specimens from the respiratory tract, and compared this medium with blood agar with S.aureus streak.Results GI of Hin on ICCA was 17.8, which was hi...
出处
《中华检验医学杂志》
CAS
CSCD
1998年第3期32-34,共3页
Chinese Journal of Laboratory Medicine
关键词
嗜血杆菌属
培养基
呼吸道感染
Haemophilus influenzae Media Respiratory tract infections
