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强化海藻酸钠凝胶制备固定化酶 被引量:14

Preparation of Immobilized Enzyme by Strengthened Na alginate Agglutination
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摘要 为了确定生产帕拉金糖(异麦芽酮糖)的固化酶最佳条件,在固化材料和固化方法上进行多项试验。结果表明:使用添加剂强化海藻酸钙凝胶包埋整个细胞酶,固化效果最佳,固化酶的酶活为30~60u/g,蔗糖平均转化率85%,最高转化率95%,实验室中固化酶连续转化半衰期长达45d。 In order to determine the optimum immobilized conditions for producing palatinose, the material and method of immobilized enzyme were investigated. Experimental results showed that adding stuffed strong Ca alginate agglutination was most effective for immobilization. The enzyme activity of immobilized enzyme was 30-60 u/g. The average conversion rate of sucrose was 85% and the highest was 96%. The half life of immobilized enzyme was 45 days.
出处 《工业微生物》 CAS CSCD 1998年第3期17-20,25,共5页 Industrial Microbiology
关键词 帕拉金糖(异麦芽酮糖) 固化酶 Palatinose Immobilized enzyme
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参考文献1

  • 1孙万儒.包埋法制备珠型固定化细胞[J]微生物学通报,1986(01).

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