摘要
目的探讨小肠移植患者手术前后及发生急性排斥反应时血清瓜氨酸的水平及其意义。方法以异硫氰酸苯酯(PITC)作为柱前衍生剂,使用高效液相色谱法,测定33名正常成人血清瓜氨酸水平以作对照,同法对2例小肠移植患者手术前后及发生排斥反应前后的血清瓜氨酸水平进行动态监测。结果33名正常成人的血清瓜氨酸水平为(16.87±5.97)μmol/L。小肠移植患者术前的瓜氨酸水平均较低(<10μmol/L),移植后瓜氨酸水平升高,并维持在较高水平,患者A最高超过30μmol/L,患者B最高超过50μmol/L,发生排斥反应后,患者A的血清瓜氨酸水平迅速下降至接近术前水平,患者B的血清瓜氨酸水平低于术前。结论小肠移植患者术后血清瓜氨酸水平与排斥反应相关,但有待更多病例验证。
Objective To investigate the level of serum citrulline and its significance in the patients subject to intestinal transplantation. Methods Phenylisothiocyanate served as derivative reagent and the serum citrulline was determined by HPLC. Thirty-three healthy adults served as controls. Serum citrulline was measured in 2 cases of intestinal transplantation during different transplant periods. Results The serum citrulline level in 33 healthy adults was (16.87±5.97)μmol/L. Before operation, it was low (less tha...
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2005年第7期430-432,共3页
Chinese Journal of Organ Transplantation
基金
国家重点基础研究发展规划(2003CB515502)
关键词
移植
小肠
瓜氨酸
色谱法
高压液相
Transplantation
Intestine
small
Citrulline
Chromatography
high pressure liquid
