摘要
目的:对黄芪药材实际可提供黄芪甲苷的含量进行研究和测定。方法:通过对三种处理方法的比较,选择氢氧化钾甲醇溶液皂化的方法;采用HPLC-ELSD法作为测定方法,以Irregular C18(4.6 mm×250 mm,5μm)为色谱柱,乙腈-水(32∶68)为流动相,流速1.0 ml/min,漂移管温度为100℃,气流量为2.7 L/min。结果:分别对不同产地的八批黄芪药材进行了测定,并对该测定方法进行了稳定性、精密度及重复性等方法学考察,结果符合要求。结论:不同处理方法黄芪药材中黄芪甲苷的含量与药典法所测得的黄芪甲苷的含量之间差异显著。
Objective: To determine the total content of astragaloside Ⅳ in Radix Astragali.Methods: The measurement conditions were used as follows: Irregular C18(4.6 mm×250 mm,5 μm) column;mobile phase: acetonitrile-water(32∶68);flow rate: 1.0 ml/min;detector: ELSD 2000;the temperature of drift tube: 100℃;gas flow: 2.7L/min.Results: Eight batches of Radix Astragali from different sources were determined.The stability,precision and reproducibility of the method were studied,RSD<3%.Conclusion: There is great difference...
出处
《中药材》
CAS
CSCD
北大核心
2008年第3期385-387,共3页
Journal of Chinese Medicinal Materials
关键词
黄芪
黄芪甲苷
含量
Radix Astragali
Astragaloside Ⅳ
Content
