摘要
为了解决种苗检疫、抗病性早期鉴定等基本问题,为葡萄无毒化栽培的提供基本保障。本实验从感染葡萄病毒B的葡萄皮层中提取总RNA,以其为模板进行一步RT-PCR扩增,并利用此扩增产物为模板进行二次扩增,二者均能获得与预期片段大小一致长约460bp和243bp的扩增产物,在此基础上,建立了萄病毒B的一步RT-PCR、巢式PCR以及斑点杂交优化检测体系。利用本实验所建立的优化检测体系对葡萄样本进行检测,表现出了良好的特异性与稳定性。
To resolve the problems of the quarantine of young plant and identification to resistance in early period and provide a guarantee to virus-free culture in grapevine.The total RNA,extracted from grapevine virus B(GVB)-infected grapevine phloem tissue,was used for the amplification of GVB by one-tube reverse transcript polymerase chain reaction (RT-PCR).About 460bp fragments of GVB have been obtained based on the optimized system.The PCR products were used as template for amplification by nest PCR and about 2...
出处
《石河子大学学报（自然科学版）》
CAS
2007年第3期282-285,共4页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(30060053
30360066)
新疆兵团科委项目(NKB02SDXNK01SW)
关键词
葡萄病毒B
扩增
斑点杂交
特异性
稳定性
grapevine virus B（GVB）
amplification
dot-blot
specificity
stability
