摘要
为了获得安全性较好的生长抑素(SS)基因疫苗,采用PCR扩增pcS/SS中的SS基因,将其融合到pUSS/S质粒S基因后,构建pUS/2SS质粒;然后将S/2SS融合基因亚克隆到pEGFP-N_1中,得到S/2SS融合表达质粒pES/2SS。酶切和测序鉴定表明:重组质粒pES/2SS构建成功。将pES/2SS转染HeLa细胞,用ELISA检测表达的融合蛋白可与SS抗体特异性结合;将pES/2SS免疫10只大鼠(每只100μg)后,5只大鼠产生抗SS抗体,而对照组未检出SS抗体。结果证明,所构建的质粒pES/2SS可在真核细胞中表达生长抑素,用于免疫可产生抗SS特异性抗体。
To improve the safety of the somatostatin(SS)gene vaccine,a new expression plasmid pES/2SS as follows were constructed.SS gene in pcS/SS was amplified by PCR and was then fused to close behind the S gene of plasmid pUSS/S to obtain the pUS/2SS plasmid.Subsequently the fused gene of S/2SS was subcloned into pEGFP-N_1 to get a S/ 2SS recombinant expression plasmid pES/2SS.These plasmids were identified by restriction endonuclease digestion and se- quencing.The result showed that the pES/2SS was constructed su...
出处
《扬州大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2007年第2期5-8,共4页
Journal of Yangzhou University：Agricultural and Life Science Edition
基金
国家自然科学基金(30270959)
关键词
生长抑素
克隆
真核表达
基因免疫
大鼠
somatostatin
gene cloning
eukaryotes expression
gene immunization
rat
