摘要
目的研究DDT类农药(p,p′-DDT、o,p′-DDT、p,p′-DDD、p,p′-DDE)的拟雌激素活性。方法用重组人雌激素受体(hER)基因酵母细胞(W303-1A-hER-ERE-LacZ)评价DDT类农药的拟雌激素活性。将终浓度为10-9～10-15mol/L的17β-雌二醇、终浓度为10-3～10-9mol/L的p,p′-DDT、o,p′-DDT、p,p′-DDD、p,p′-DDE加入酵母细胞培养液,作用4h,通过定量测定β-半乳糖苷酶的活力表达DDT类农药的雌激素作用强度。结果DDT类农药能像配体一样与hER结合,发挥雌激素效应,并具有剂量-效应关系;o,p′-DDT雌激素活性较强,EC50为5.30×10-7mol/L,p,p′-DDE雌激素活性较弱,EC50为9.28×10-5mol/L,同分异构体间(o,p′-DDT、p,p′-DDT)雌激素活性具有协同效应,联合效应相加指数(AI)为1.5;同系物间(p,p′-DDD、p,p′-DDE)雌激素活性具有拮抗效应,AI=-1.38;4种DDT类农药联合雌激素效应呈协同作用,回归方程为y^=2.6006x2-28.079x-21.757,EC50为2.57×10-8mol/L,AI=0.83。结论DDT类农药是经雌激素受体(ER)介导的环境雌激素,p,p′-DDT、o,p′-DDT、p,p′-DDD、p,p′-DDE联合效应拟雌激素活性呈协同激活作用。
Objective To study the estrogenic effects of DDT-derivative pesticides. Methods Using a recombinant human estrogen receptor gene yeast cell to estrogenic activity of DDT pesticides. The different concentrations of 17β-estradiol and individual DDT isomers and homologue such as p,p′-DDT, o,p′-DDT, p,p′-DDD and p,p′-DDE and their mixtures were added to yeast cultures, then estrogenic activity was measured by quantification of β-galactosidase after 4 hours culture. Results DDT showed the ability to play the estrogenic effects like a ligand to bind hER and the dose dependent model existed between the estrogenic effects and the DDT concentration. The relative activity of o,p′-DDT, EC50=5.30×10-7mol/L, was higher and the estrogenic effects of isomers exhibited synergism, AI=1.5. The relative activity of p,p′-DDE , EC50=9.28×10-5mol/L, was lower and the antagonistical action between the homologues was found, AI=-1.38. DDT pesticides presented a synergism, the regression equation was =-2.600 6x2-28.079x-21.757,EC50 was 2.57×10-8mol/L, AI=0.83. Conclusion DDTs are ER mediated environmental estrogens, p,p′-DDT, o,p′-DDT, p,p′-DDD and p,p′-DDE show a synergic effect.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第9期664-669,共6页
Journal of Environment and Health
基金
国家教育部留学回国人员科研启动基金(PK0407149)
江苏省教育厅自然科学基金(03KJB610168)
江苏省卫生厅医学科技发展基金(Z200328)
关键词
农药
酵母细胞
人雌激素受体
联合效应
Pesticides
Yeast cell assay
Human estrogen receptor
Joint effects
