摘要
目的探讨氟对原代大鼠海马细胞存活、细胞周期、凋亡和核因子-κB(NF-κB)表达的影响。方法原代培养大鼠海马细胞分别暴露于20、40和80μg/ml氟化钠24h后,采用噻唑蓝(MTT)、流式细胞术(FCM)、逆转录PCR(RT-PCR)分别检测大鼠海马细胞存活情况、细胞周期分布、凋亡率、NF-κB表达水平。结果与对照组比较,80μg/ml剂量组海马细胞存活率下降(P<0.05);40、80μg/ml剂量组凋亡率和S期细胞百分率皆显著高于对照组(P<0.05),各剂量组NF-κBmRNA显著高于对照组(P<0.05)。结论一定剂量氟可抑制海马细胞存活,诱导NF-κBmRNA表达和凋亡发生。氟对发育中海马细胞NF-κB表达水平的影响可能是氟神经毒性的机制之一。
Objective To investigate the effects of fluoride on the cell viability, cell cycle, apoptosis, and expression levels of NF-κB in primary cultured rat hippocampal neurons. Methods The rate of cell survivor, distribution of cell cycle, the percentage of apoptotic cells and the mRNA expression level of NF-κB were respectively measured by methyl thiazolyl tetrazolium (MTT), flow cytometry (FCM), and reverse transcriptase polymerase chain reaction (RT-PCR) after the hippocampal neurons were incubated with 20, 40, and 80 μg/ml sodium fluoride for 24 hours in vitro. Results Compared with the control group, the rate of cell survivor were significantly decreased in 80 μg/ml fluoride-treated group (P<0.05). Fluoride of 40 and 80 μg/ml doses increased the percentages of S phase and apoptotic cells as compared to the controls (P<0.05, P<0.05, respectively). Compared with the control group, the mRNA expression level of NF-κB in all fluoride-treated groups were markedly enhanced (P<0.05). Conclusion Fluoride can restrain the survival of rat hippocampal neurons, induce apoptosis and NF-κB mRNA expression. The altered expression of NF-κB in developmental hippocampus may be one of the neurotoxic mechanisms induced by fluoride.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第9期669-671,共3页
Journal of Environment and Health
基金
国家自然科学基金资助项目(30371250
30671819)
关键词
氟
海马细胞
细胞周期
凋亡
核因子ΚB
Fluoride
Hippocampal neuron
Cell cycle
Apoptosis
Nuclear factor kappa B
