摘要
目的探讨氟铝联合染毒对小鼠肝脏的毒性效应及其可能机制。方法将清洁级昆明种小鼠随机分为对照组(自来水)、低氟(LF)组(氟含量为50mg/L)、低铝(LAl)组(铝含量为200mg/L)、高氟(HF)组(氟含量为150mg/L)、高铝(HAl)组(铝含量为600mg/L)、低氟低铝(LF+LAl)组(氟含量为50mg/L,铝含量为200mg/L)、低氟高铝(LF+HAl)组(氟含量为50mg/L,铝含量为600mg/L)、高氟低铝(HF+LAl)组(氟含量为150mg/L,铝含量为200mg/L)、高氟高铝(HF+HAl)组(氟含量为150mg/L,铝含量为600mg/L),每组雌、雄各8只,自由饮用相应浓度的含氟和(或)铝的水,24周后,检测肝功能(血清丙氨酸和天门冬氨酸氨基转移酶活力、总胆红素、直接胆红素、间接胆红素、白蛋白)和血清中钙、磷、镁的含量。结果不同剂量单独染氟及氟铝联合染毒时各组间肝功能指标差异有统计学意义(氟单独染毒:F=2.61,P<0.01;氟铝联合染毒:F=3.12,P<0.01),不同剂量单独染铝时肝功能指标改变无统计学意义(F=1.73,P>0.05)。除HF+HAl组外,LF组、LAl组、HF组、HAl组、LF+LAl组,LF+Hal组,HF+LAl组血清钙含量均低于对照组。各组血磷、血镁含量间,差异均无统计学意义(均P>0.05)。结论氟、铝联合染毒对肝脏存在毒性,高氟高铝联合染毒较单独染毒时表现为拮抗作用,这可能与其干扰体内钙的代谢有关。
Objective To study the combined toxic effects of fluoride and aluminum on the liver of mouse and the probable mechanism. Methods Kunming mice were randomly divided into nine groups and treated with fluoride and aluminum through drinking water:control(tap water)group, 50 mg/L fluoride group, 150 mg/L fluoride group, 200 mg/L aluminum group, 600 mg/L aluminum group, 50 mg/L fluoride+200 mg/L aluminum group, 50 mg/L fluoride+600 mg/L aluminum group, 150 mg/L fluoride+200 mg/L aluminum group, 150 mg/L fluoride+600 mg/L aluminum group. After 24 weeks of treatment, the activity of ALT, AST, the content of TBIL, DBIL, IBIL, ALB, Ca, P and Mg in the serum was determined. Results Different doses of single fluoride or fluoride+aluminum led to significant different changes in the liver function(single fluoride:F=2.61, P<0.01 and fluoride+aluminum:F=3.12, P<0.01), no significant changes were seen in aluminum exposed groups(F=1.73, P>0.05). Compared with the control group, the Ca level in the serum decreased in the exposed groups except 150 mg/L fluoride+600 mg/L aluminum group(P<0.05). The P and Mg levels did not significantly change in each group(P>0.05). Conclusion The combination of fluoride and aluminum at certain levels has a toxic effect on the liver, but the combination of fluoride and aluminum at high doses shows an antagonistic effect.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2007年第11期857-860,共4页
Journal of Environment and Health
基金
贵州省科技厅基金资助项目(2004-3052)
关键词
氟化物
铝
肝脏
Fluorides
Aluminum
Liver
