摘要
应用PCR技术对1株鸵鸟源新城疫病毒TN株融合蛋白基因(F基因)进行扩增,将其克隆到pMD18-T载体后进行序列测定,并与国内外NDV毒株对应序列进行比较分析.结果表明,TN株F基因的长度为1 662 bp,可编码553个氨基酸,其裂解位点的氨基酸序列为112G-R-Q-G-R-L117,为1株新城疫弱毒株;与贵州省其他禽源(包括肉鸡、蛋鸡、越南斗鸡、七彩山鸡和鸽子等)毒株间的核苷酸同源性为84.0%～89.6%,氨基酸同源性为87.5%～92.1%;与国内外NDV代表株(Lasota株、B1株、F48E9株、CH2000株和TW2000株)的核苷酸同源性为84.4%～98.6%,氨基酸同源性为88.3%～98.0%.经系统发生树分析,TN株与NDV弱毒株Lasota株和B1株在同一分支上,属于基因II型NDV.
Full-length F protein gene of the TN strain of newcastle disease virus(NDV) isolated from ostrich in Guizhou province was amplified by using reverse transcription polymerase chain reaction(RT-PCR) and cloned into pMD18-T vector,then sequenced and analyzed with reference NDV strains in GenBank.The results showed that the sequence of F protein gene of TN strain was determined with its full-length of 1 662 bp coding 553 amino acids.The amino acid sequence in the cleavage site of the deduced peptide is 112G-R-Q-G-R-L117.The results of sequence analysis of F protein gene in full-length indicated that the homology between TN strain and other NDV isolated from different avains in Guizhou province would be 84.0%～99.7% in nucleotide sequence and 87.5%～99.3%in amino acid sequence,and the homology of this strain with the representative NDV strains in internal and overseas would be 84.2%～98.9% and 87.2%～98.6% respectively.The phylogenetic analysis showed that TN strain belonged to II genotype of NDV.
出处
《中国兽药杂志》
2007年第12期16-19,共4页
Chinese Journal of Veterinary Drug
基金
贵州省自然科学基金项目[黔科合J字(2005)2030号]
贵州大学人才科研基金项目(贵大人基合字[2005]18号)
关键词
鸵鸟
新城疫病毒
融合蛋白基因
序列分析
ostrich
newcastle disease virus(NDV)
F protein gene
sequence analysis
