摘要
目的探讨ALOX5AP基因突变与缺血性脑卒中的关系。方法采用聚合酶链反应-单链构象多态性法及双脱氧末端终止法分析26例急性脑梗死患者及23名正常对照者的ALOX5AP基因的单核苷多态性(SNPs)。结果脑梗死组出现ALOX5AP基因SG13S100杂合型(A/G)(18例,69.2%)的频率显著高于正常对照组(8人,34.8%)(P<0.05)。男性脑梗死患者ALOX5AP基因SG13S100杂合型(A/G)的频率(86.7%)明显高于女性患者(45.5%)(P<0.05)。结论ALOX5AP基因突变与缺血性脑卒中的发生有关,急性脑梗死患者ALOX5AP基因SG13S100的突变率显著增高,其中男性患者尤为明显。
Objective To investigate the association between the mutations in the ALOX5AP gene and ischemic stroke.Methods In 26 patients with acute cerebral infarction and 23 normal controls,the mononucleoside polymorphism(SNPs)in the ALOX5AP was analysised by the single strand conformation polymorphism analysis of polymerase chain reaction products(PCR-SSCP)and Sanger's dideoxy chain termination.Results The frequency of SG13S100(A/G)in the ALOX5AP gene in group of cerebrall infarction(18/26,69.2%)was significantly higher than that in normal controls(8/23,34.8%)(P<0.05).And it was obviously higher in male patients(86.7%)than that in female(45.5%)(P<0.05).Conclusion The ALOX5AP gene is associated with ischemic stroke.The mutation rate of SG13S100(A/G)in the ALOX5AP of patients with acute cerebral infarction increase significantly,especially in male patients.
出处
《临床神经病学杂志》
CAS
北大核心
2007年第5期326-328,共3页
Journal of Clinical Neurology
