摘要
构建猪传染性胸膜肺炎放线杆菌(APP)apxIA基因的真核表达载体pPICZαA/apxI,并在毕赤酵母GS115中进行表达。SDS-PAGE显示仅在浓缩40倍的上清中检测得到表达产物,同时经RT-PCR可检测到重组酵母中编码目的基因成熟肽的mRNA,经分析发现目的序列AT含量高达62%,其中含49个毕赤酵母稀有密码子,占15.4%。证实ApxIA在毕赤酵母中为低水平表达。
A recombinant eukaryotic expression vector of apxIA gene from Actinobacillus pleuropneumoniae (App), named pPICZαA/apxI, was constructed and the apxIA gene was expressed in Pichia pastoris. SDS-PAGE analysis revealed that the recombinant ApxIA protein could be tested in the concentrated supernatant, and recognized by positive serum against APP serotype 1 using western-blot. The mRNA coding the ApxIA could be tested by RT-PCR in the recombinant yeast. In the interest sequence, the AT% was up to 62% and there...
出处
《畜牧与兽医》
北大核心
2008年第4期19-23,共5页
Animal Husbandry & Veterinary Medicine
基金
广西科技攻关项目资助(桂科攻0537008-3B)
广西区水产畜牧局科研计划(桂渔牧科061910)
