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肿瘤坏死因子对谷氨酰胺促大鼠组织蛋白质合成的影响 被引量：1

The effects of TNF on the glutamine-enhanced protein synthesis in rats' tissues
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摘要 目的:研究肿瘤坏死因子(TNF-α)对谷氨酰胺(Gln)促大鼠组织蛋白质合成的影响。方法:将30只SD大鼠随机分为TPN(对照)组、Gln组和TNF-α组。所有大鼠均行3 d的TPN。对照组仅给予普通TPN液;Gln组在PN液中添加丙氨酰谷氨酰胺二肽,Gln剂量为0.3 g/(kg.d),共72 h;TNF-α组与Gln组相同,并在第3天持续24 h静脉滴注5μg/(kg.d)的TNF-α。所有大鼠均在取材前30 min,一次性静脉注射1.0 mmol/kg的L-15N亮氨酸。实验结束时,分别测定血浆TNF-α、Gln浓度及骨骼肌、小肠、肝组织的Gln浓度和蛋白质合成率。结果:TNF-α组血浆TNF和Gln浓度以及骨骼肌、小肠、肝组织Gln浓度,均显著高于其他两组;Gln组和TNF-α组骨骼肌、小肠、肝组织中蛋白质合成率均高于对照组,Gln组最高。各组间差异均有显著性意义(P<0.01)。结论:Gln能促进组织蛋白质合成,TNF可抑制感染状态下Gln的促蛋白质合成作用。Gln和TNF均能使血浆及组织中Gln浓度升高。 Objective:To determine whether the regulation of glutamine on protein synthesis in tissues could be inhibited by TNF-α(one of the major mediators) in sepsis in a rat model. Methods:Thirty male Sprague-Dawley rats were randomly assigned into three groups:TPN(control),Gln(treated with glutamine),and TNF-α group(treated with glutamine and TNF-α).All rats received isonitrogenous and isocaloric TPN solutions for 3 days.Control group was supplemented by TPN;glutamine group was given glutamine-enriched TPN and TNF...
作者 周济宏 李幼生 曹亚澄 黎介寿
机构地区 南京大学医学院临床学院南京军区南京总医院解放军普通外科研究所 中国科学院土壤研究所
出处 《肠外与肠内营养》 CAS 2008年第1期7-10,共4页 Parenteral & Enteral Nutrition
基金 国家自然科学基金资助项目(30672060)
关键词 全肠外营养 谷氨酰胺 肿瘤坏死因子 稳定性核素 蛋白质合成率 大鼠 Stable isotopes L-15N Leucine Fractional synthesis rate Mass spectrometry Glutamine TNF-α
分类号 R363 [医药卫生—病理学]
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参考文献30

  • 1[1]Wilmore DW,Shabert JK.Role of glataminc in immunologic responses[J].Nutrition,1998,14(7-8):618-626.
  • 2[2]Novak F,Heyland DK,Avenell A,et al.Glutamine supplementation in serious illness:a systematic review of the evidence[J].Crit Care Med,2002,30(9):2022-2029.
  • 3[3]DSouza R,PoweH-Tuck J.Glutamine supplements in the critically ill[J].J R Soc Med,2004,97(9):425-427.
  • 4[4]Melis GC,ter Wengel N,Boelens PG,et al.Glutamine:recent developments in research on the clinical significance of glutamine[J].Curr Opin Clin Nutr Metab Care,2004,7(1):59-70.
  • 5[5]Goetem C,Wenn A,Mertes N,et al.Parenteral L-alanyl-L-glutamine impmves 6-month outcome in critically ill patients[J].Crit Care Med,2002,30(9):2032-2037.
  • 6[6]Garcia-de-Lorenzo A,Zarazaga A,Gareia-Luna PP,et al.Clinical evidence for enteral nutritional support with glutamine:a systematic review[J],Nutrition,2003,19(9):805-811.
  • 7[7]OLeary MJ,Fergusan CN,Renme M,et al.Effect of growth hormone on mosele and liver protein synthesis in septic rats receiving glutamine-enriched parenteral nutrition[J].Crit Care Med,2002,30(5):1099-1105.
  • 8[8]Gore DC,Wolfe RR.Glutamine supplementation fails to affect muscle protein kinetics in critically ill patients[J].JPEN,2002,26(6):342-349.
  • 9[9]Wisehmeyer PE,Riehm J,Singleton KD,et al.Glutamine attenuates tumor necrosis factor-alpha release and enhances heat shock protein 72 in human peripheral blood mononuclear cells[J].Nutrition,2003,19(1):1-6.
  • 10周济宏,李幼生,曹澄亚,朱明芳,韩勇,黎介寿.L-^(15)N亮氨酸和质谱测定大鼠肝蛋白质合成[J].肠外与肠内营养,2005,12(3):133-136. 被引量：6

二级参考文献27

  • 1周济宏,李幼生,黎介寿.稳定同位素在蛋白质合成率测定中的应用[J].肠外与肠内营养,2005,12(1):46-49. 被引量：10
  • 2周济宏,李幼生,曹澄亚,朱明芳,韩勇,黎介寿.L-^(15)N亮氨酸和质谱测定大鼠肝蛋白质合成[J].肠外与肠内营养,2005,12(3):133-136. 被引量：6
  • 3刘放南,李元新.微量凯氏定氮法在外科营养支持中的应用[J].肠外与肠内营养,1995,2(3):199-200. 被引量：10
  • 4仲宁，色谱，1994年，5卷，369页
  • 5Garlick PJ, McNurlan MA, Essen p,et al. Measurement of tissure protein synthesis rates in vivo: a critical analysis of contrasting methods[J]. Am J Physiol, 1994, 266 (3 Pt 1) : E 287-297.
  • 6Rennie M J, Smith k, Watt PW. Measurement of human tissue protein synthesis: an optimal approach[J] . Am J Physiol, 1994,266(3 Pt 1 ) : E298-E307.
  • 7Garlick PJ, Caso G, Mynarcik D,et al. Nutritional regulation of protein metabolism [J]. J Anim Sci, 2002, 80 ( Suppl 2 ) : E135-142.
  • 8Parks E J, Matthews DE. A.S.P.N. 2003 Resarch workshop on using tracer to measure carbohydrate, Fat, and amino acid metabolism in humans [J]. JPEN, 2004, 28 (1) :38-54.
  • 9Schwenk WF, Tsalikian E, Beaufrere B,et al. Recycling of an amino acid label with prolonged isotope infusion: implications for kinetic studies[J]. Am J physiol, 1985,248(4 Pt 1) :E482-487.
  • 10Smith K, Barua JM, Watt PW,et al. Flooding with leucine stimulates human muscle protein incorporation of continuously infuse L-[1-^13C] valine[J]. Am J physiol, 1992, 262 (3 Pt 1):E372-376.

共引文献21

同被引文献34

  • 1周济宏,胡心宝,洪志坚,李幼生,黎介寿.应用RNA干扰技术检测ASCT2功能[J].医学研究生学报,2011,24(11):1124-1128. 被引量：4
  • 2Esper AM, Martin GS. Extending international sepsis epidemiolo- gy :the impact of organ dysfunction. Crit Care,2009,13 ( 1 ) : 120.
  • 3Tjader IBerg AWemerman J. Exogenous glutamine-compensating a shortage? Crit Care Med ,2007,35 ( 9 Suppl) : S553-556.
  • 4Cohen J, Chinw D. Nutrition and sepsis. World Rev Nutr Diet, 2013,105 : 116-125.
  • 5Kim M, Wischmeyer PE. Glutamine. World Rev Nutr Diet, 2013, 105:90-96.
  • 6Coeffier M, Dechelotte P. The role of glutamine in intensive care unit patients: mechanisms of action and clinical outcome. Nutr Rev, 2005,63 ( 2 ) :65-69.
  • 7Anbar R. Enteral nutrition. World Rev Nutr Diet,2013,105:50- 58.
  • 8Wischmeyer PE. Glutamine: the first clinically relevant pharma- cological regulator of heat shock protein expression? Curr Opin Clin Nutr Metab Care,2006,9(3) :201-206.
  • 9Niederlechner S, Baird C, Petrie B, et al. Epidermal gowth factor receptor expression and signaling is essential in glutamine's tyro- protective mechanism in heat-stressed intestinal epithelial-6 cells. Am J Physiol Gastrointest Liver Physiol,2013,304 (5): G543- G552.
  • 10Singleton KD, Beckey VE, Wischmeyer PE. Glutamine prevents activation of NF- kappaB and stress kinase pathways, attenuates inflammatory cytokine release, and prevents acute respiratory dis- tress syndrome (ARDS) following sepsis. Shock, 2005,24 ( 6 ) : 583-589.

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肠外与肠内营养
2008年 第1期

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