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流式细胞仪检测网织红细胞百分率及网织红细胞成熟指数的意义探讨 被引量:3

Reticulocyte percentage and reticulocyte maturity index determined by flow cytometry.
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摘要 目的建立本地区健康成年人群网织红细胞百分率及网织红细胞成熟指数的正常参考值,同时与贫血患者群进行比较。方法采用流式细胞术,利用荧光染料噻唑橙(TO)标记。结果健康成年人群网织红细胞的百分率为1.14±0.23,网织红细胞成熟指数为8.08±1.95;贫血患者群网织红细胞的百分率为4.13±2.91,网织红细胞成熟指数为19.16±8.37,两者相比较差异有统计学意义(P<0.05)。结论利用流式细胞仪进行网织红细胞百分率及网织红细胞成熟指数的检测,快速、客观、准确,可作为临床检验指标之一。 Objective To establish the normal range of reticulocyte percentage and reticulocyte maturity index in healthy people and to compare it with anemia patients.Methods Flow cytometric analysis was used and thiazole orange was taken as marker.Results Reticulocyte percentage and reticulocyte maturity index were 1.14±0.23 and 8.08±1.95 in healthy people respectively,4.13±2.91 and 19.16±8.37 in anemia patients respectively.There was significant difference between normal people and anemia patients.Conclusion Flow cytometry determining reticulocyte percentage and reticulocyte maturity index seems to be rapid,objective and precise method,and appropriate for clinical detection.
出处 《检验医学与临床》 CAS 2006年第5期198-199,共2页 Laboratory Medicine and Clinic
关键词 流式细胞术 网织红细胞百分率 网织红细胞成熟指数 Flow cytometry Reticulocyte percentage Reticulocyte maturity index
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参考文献2

  • 1[2]Bruch HD,Michael DC,Nancy CB,et al.Proposal for standardization of flow cytometric reticulocyte maturity index(RMI) measurements[J].Cytometry,1993,14(3):318-326.
  • 2[3]Davis BH,Ornvold K,Bigelow NC.Flow cytometric reticulocyte maturity index:a useful laboratory parameter of erythropoietic activity in anemia[J].Cytometry,1995,22(1):35-39.

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