3[3]Livak KJ,Flood SJ,Marmaro J,et al.Oligonucleotides with fluorescent dyes at opposite ends provide a quenched probe system useful for detecting PCR product and nucleic acid hybridization[J].PCR Methods Appl,1995,4(6):357-362.
4[4]Cindy AE,Kathleen DD,Kazuyuki K,et al.CpG island hypermethylation in human colorectal tumors is not associated with DNA methyltransferase overexpression[J].Cancer Res,1999,59:2302-2306.
6[6]Cindy AE,Kathleen DD,Kazuyuki K,et al.MethyLight:a high-throughput assay to measure DNA methylation[J].Nucleic Acids Res,2000,28(8):32-40.
7[7]James GH,Jeremy RG,Sanna M,et al.Methylation-specific PCR:a novel PCR assay for methylation status of CpG islands.Proc[J].Natl Acad Sci USA,1996,93 (18):9831-9836.
8[8]Wong IH,Zhang J,Lai PB,et al.Quantitative analysis of tumor-derived methylated p16INK4a sequences in plasma,serum,and blood cells of hepatocellular carcinoma patients[J].Clin Cancer Res,2003,9(3):1047-1052.
9[9]Zeschnigk M,Bohringer S,Price EA,et al.A novel realtime PCR assay for quantitative analysis of methylated alleles (QAMA):analysis of the retinoblastoma locus[J].Nucleic Acids Res,2004,32(16):125.
10[10]Leung WK,To KF,Chu ES,et al.Potential diagnostic and prognostic values of detecting promoter hypermethylation in the serum of patients with gastric cancer[J].Br J Cancer,2005,92(12):2190-2194.
2Lie YS;Petropoulos CJ.Advances in quantitative PCR technology:5' nuclease assays[J],1998(01).
3Giulietti A;Overbergh L;Valckx D.An overview of real-time quantitative PCR: applications to quantify cytokine gene expression,2001(04).
4Heid CA;Stevens J;Livak K J.Real time quantitativePCR[J],1996(06).
5Yin JL;Shackel NA;Zekry A.Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) for measurement of cytokine and growth factor mRNA expression with fluorogenic probes or SYBR Green I[J],2001(03).
6Tyagi S;Bratu DP;Kramer FR.Multicolor molecular beacons for allele discrimination[J],1998(01).
7Whitcombe D;Theaker J;Guy SP.Detection of PCR products using self-probing amplicons and fluorescence[J],1999(08).
8Nazarenko IA;Bhatnagar SK;Hohman RJ.A closed tube format for amplification and detection of DNA based on energy transfer[J],1997(12).
9Wittwer CT;Herrmann MG;Moss AA.Continuous fluorescence monitoring of rapid cycle DNA amplification[J],1997(01).
10Walker NJ.Real-time and quantitative PCR: applications to mechanism-based toxicology[J],2001(03).