摘要
目的检测磷脂酰肌醇3激酶-视网膜母细胞瘤基因(p55PI3K-Rb)信号传导途径在胃肠道正常组织、癌旁组织及癌组织中的表达,并阐述其意义。方法收集20例胃肠道肿瘤患者(胃癌6例,结肠癌5例,直肠癌9例)手术切除标本,每例均包括癌组织、相应的癌旁组织及正常组织。应用免疫沉淀(Rb单克隆抗体)和Western blot方法(p55PI3K多克隆抗体)检测p55PI3K与Rb在组织中的相互作用。结果8例胃肠道肿瘤标本(胃癌2例,结肠癌2例,直肠癌4例,均为腺癌)Rb与p55PI3K相互作用为阳性,阳性率为40%(8/20),且正常组织、癌旁组织及癌组织三者之间p55PI3K-Rb作用强弱差异无显著性意义(P>0.05)。结论在胃肠道肿瘤形成过程中,p55PI3K-Rb信号传导通路是存在的。通过阻断p55PI3K-Rb通路可能阻断肿瘤的发生,有望为临床胃肠道肿瘤的治疗开辟一条新的途径。
Objective To detect the interaction of p55PI3KRb in the gastrointestinal normal mucosa,gastrointestinal cancers and corresponding paraneoplastic tissues,and to elucidate its significance.Methods Twenty cases of gastrointestinal normal mucosa,gastrointestinal cancers and paraneoplastic tissues were collected.Immunoprecipitation and Western blot were employed to detect the expression of p55PI3K and Rb as well as the interaction of p55PI3K-Rb respectively.Results p55PI3K-Rb interaction was positive in 8 cases of gastrointestinal tumor specimens(all gastric adenocarcinoma).And the interaction of p55PI3K-Rb had no significant difference in tissues of gastrointestinal normal mucosa,paraneoplastic and gastrointestinal cancers(P>0.05).Conclusion Interaction of p55PI3K-Rb exists in gastrointestinal tumors.It is possible to interrupt tumors genesis by blocking the interaction of p55PI3K-Rb,which may provide a new treatment pathway for gastrointestinal cancers.
出处
《华中科技大学学报（医学版）》
CAS
CSCD
北大核心
2006年第6期761-763,封3,共4页
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
基金
国家自然科学基金资助项目(No.30300338)
