摘要
目的:建立活络油中有效成分α-蒎烯、桉油精、樟脑、薄荷脑、水杨酸甲酯、桂皮醛、丁香酚的气相色谱测定法。方法:色谱条件为DB-1毛细管色谱柱,柱温90℃,保持1 min后,以6℃.min-1升至180℃后,继续以25℃.min-1升至250℃,保持5min,载气为氮气。进样口温度为220℃,检测器温度为260℃,FID检测器,无分流进样。结果:α-蒎烯的线性范围6.94~41.64μg.mL-1,桉油精的线性范围1.00~6.01μg.mL-1,樟脑的线性范围6.86~41.18μg.mL-1,薄荷脑的线性范围24.09~144.55μg.mL-1,水杨酸甲酯的线性范围24.16~144.98μg.mL-1,桂皮醛的线性范围1.03~6.17μg.mL-1,丁香酚的线性范围1.04~6.25μg.mL-1,RSD分别为1.8,1.1,0.9,0.7,1.1,1.7,1.1%。结论:方法简便、准确,可用于活络油的质量控制。
Objective:To develop a Capillary GC method to determine the seven components,α-pinene,eucalyptol,camphor,menthol,methyl salicylate,cinnamia aldehyde and eucalyptal in Huoluo oil.Method:A fused silica capillary column was used.The intial temperature of column was kept at 90℃ for 1 min,then raised to 180℃ at the rate of 6℃·min^(-1),finally to 250℃ at the rate of 25℃·min^(-1) and maintained the temperature for 5 min,the carry gas was nitrogen.The injector and detector temperature was 220℃ and 260℃.Injections were performed in the splitless mode and an FID was used as the detector.Results:The standards curves of α-pinene,eucalyptol,camphor,menthol,methyl salicylate,cinnamia aldehyde,eucalyptal are linear within the range of 6.94～41.64μg·mL^(-1),1.00～6.01 μg·mL^(-1),6.86～41.1 μg·mL^(-1),24.1～144.5 μg·mL^(-1),24.09～144.55μg·mL^(-1),24.16～144.98μg·mL^(-1),1.03～6.17μg·mL^(-1),1.04～6.25μg·mL^(-1),and RSD was 1.8,1.1,0.9,0.7,1.1,1.7,1.1%.Conclusion:This method is simple and accurate,and can be used to control of Huoluo oil.
出处
《中国实验方剂学杂志》
CAS
2006年第10期16-18,共3页
Chinese Journal of Experimental Traditional Medical Formulae
