摘要
目的:观察脾虚证模型大鼠造模不同时间点血中胃肠激素含量的变化.方法:采用游泳劳倦加饮食失节法,大鼠连续造模3w,自然恢复1w.结果:与对照组比较,模型大鼠在造模2～3w出现明显饮食减少、大便溏薄、活动减少、倦卧拱背等改变;体重显著降低,第4w仍未见明显恢复;尿D-木糖排泄率在第2,3,4w均显著降低;血清胃泌素(GAS)和胃动素(MTL)水平第3w显著降低,自然恢复期显著升高;生长抑素水平于造模1w后明显降低,其余各点无明显变化;血管活性肠肽(VIP)水平2w时明显上升,第3w显著降低;P物质(SP)水平3w时显著降低;神经肽Y(NPY)水平于2,3w时明显降低,β-内啡肽(β-EP)水平于4w时明显降低.结论:该病因造模法造模3w可引起大鼠食少便溏、倦怠少动等表现,同时体重降低、尿D-木糖排泄率降低以及伴有多种胃肠激素的异常变化.
Objective:To observe gastroenteric hormones content changes of blood in model rat of spleen-qi deficiency during different modelling time points.Methods:Adopting excess fatigue(by swimming) and out of constant diet on rats for constant three weeks and stopping doing on them in the 4w.Results:Compared with the control group,the model rats distinctly appear diet reduction,feces rarefaction,reduction activities,accidie and extrados,etc in modelling 2～3w;weight significantly failed,and it has yet to come back in the 4w;urine D-xylose excretion rate significantly falled during 2～4w;GAS and MTL level of serum failed in the 3w and significantly came back during natural recovery period;SS level markedly lower in the 1w,and no significant changes in the remaining time points;VIP level markedly rised in the 2w,and significantly failed in 3w;SP level clearly failed in the 3w;NPY level clearly lower in the 2～3w,and β-EP level clearly declined in the 4w.Conclusion:The model rat made by excess fatigue and out of constant diet for constant three weeks can appear diet reduction,feces rarefaction,reduction activities,etc;and abnormal changes of weight,urine D-xylose excretion rate,and gastroenteric hormones of Blood.
出处
《中国实验方剂学杂志》
CAS
2006年第10期31-35,共5页
Chinese Journal of Experimental Traditional Medical Formulae
基金
"211"基金资助项目
关键词
脾虚证
动物模型
胃肠激素
syndrome of spleen-qi deficiency
animal model
gastroenteric hormones
