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薄层色谱分离分光光度法测定辣椒酱中苏丹红Ⅰ 被引量:6

Separation and Determination of Sudan Ⅰ in Hot Pepper Sauce by Thin Layer Chromatography and Spectrophotometry
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摘要 通过自制硅胶G板,利用薄层色谱法对辣椒酱中的苏丹Ⅰ进行分离,刮下斑点,溶解后用分光光度法测定,所得的波长和吸光度的谱图与标准溶液的谱图进行对照,从而确定辣椒样品中存在苏丹红Ⅰ。该方法简便快捷,现象明显,经济实用,结果可靠,尤其适合于基层检测机构及小工厂的有关食品检验。 Separation of Sudan Ⅰ in Hot Pepper Sauce by Thin Layer Chromatography as well as its determination by spectrophotom- etry were studied. Using the self-made silica gel G board, Sudan I was isolated by thin layer chromatography and the spot shaved from the silica gel G was dissolved and then determined by spectrophotometer. Results showed that this method can quickly determine the content of Sudan Ⅰ in hot pepper sauc. It especially suited small the food inspection and testing for company and primary examina...
出处 《现代食品科技》 EI CAS 2007年第2期84-86,共3页 Modern Food Science and Technology
关键词 薄层色谱法 辣椒酱 苏丹红 thin layer chromatography hot pepper sauce Sudan Ⅰ
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  • 1Ghanadzadeh A, Zakerhamidi M S, Tajalli H. Journal of Molecular Liquids,2004,109 : 143 - 148.
  • 2Nagase M, Osaki Y, Matsueda T. Journal of chromatography, 1989,465 (2) :434 - 437.
  • 3Pielesz A, Baranowska I, Rybak A et al. Environmental Research,2002,53:42 -47.
  • 4国家质检总局和国家标准委.食品中苏丹红染料的检测方法-高效液相色谱法[EB/OL].http://www.sac.gov.cn,2005—4—20.

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