摘要
目的观察重组人生长激素(rhGH)对大鼠急性胰腺炎(AP)的治疗并探讨其机制。方法ipE.coli复制大鼠AP模型。42只健康♀SD大鼠随机分为对照组(C组)、急性胰腺炎组(AP组)及rhGH治疗组(T组)。AP、T组再按观察时点分为1、3 d两个亚组。C组ip 15 m.lkg-1生理盐水;AP组ip 15 m.lkg-1E.coli(1×1010CFU.L-1)后立即im生理盐水;T组于ipE.coli后立即im 2.25 U.kg-.1d-1rhGH。观察胰腺的病变程度并进行评分,测定血清淀粉酶的活性,免疫组化检测胰腺组织中ICAM-1蛋白的表达及NF-κB核的阳性率。结果AP两亚组胰腺组织病理评分、血清淀粉酶活性、ICAM-1蛋白表达及NF-κB核阳性率均明显高于C组水平(P<0.05);rhGH治疗能明显降低上述指标水平,尤以T3d组降低最为显著。结论rh-GH治疗能明显减轻大鼠急性胰腺炎的损伤程度,其机制可能与降低NF-κB活化程度及ICAM-1蛋白的表达水平等有关。
OBJECTIVE To observe the effects of recombinant human growth hormone(rhGH) on acute pancreatitis(AP) in rats and investigate its possible mechanisms.METHODS E.coli was injected intraperitoneally(ip) to establish AP models.42 healthy female SD rats were randomly divided into three groups:control group(group C),injected physiologic saline(15 ml·kg-1,ip);group AP,received a bolus injection of E.coli(1×1010 CFU·L-1,15 ml·kg-1,ip) then immediately followed by intramuscular injection of physiologic saline;AP+rhGH...
出处
《华西药学杂志》
CAS
CSCD
北大核心
2008年第4期431-433,共3页
West China Journal of Pharmaceutical Sciences
