摘要
目的:分析多花黄精根状茎挥发油成分及检测其抑菌和抗肿瘤生物活性。方法:采用水蒸汽蒸馏法(SD)提取挥发油,用气相色谱-质谱(GC-MS)联用技术分析多花黄精根状茎精油的成分,生物活性检测采用杯碟法和Alamar blue法。结果:共鉴定出16个化合物,占总挥发油的95.97%,该挥发油对大肠杆菌、金黄色葡萄球菌、红酵母均有较强的抑制能力;对人肺癌细胞(NCI-H460)有显著的抑制作用,当浓度达到100μg.mL-1,抑制率达到98.08%。结论:实验结果表明多花黄精挥发油成分具有较强的生物活性,为进一步研究其药理作用提供了科学依据。
Objective:To analyse the constituents and determine the biological activity of volatile oil from the tubers of Polygonatum cyrtonema.Methods:The volatile oil was obtained by steam distillation.And its chemical constituents were analyzed by GC-MS.Biological activity of the volatile oil was screened with microbiological method and Alamar Blue method.Results:16 compounds were identified,making up 95.97% of the total contents;The volatile oil could inhibit the growth of Escherichia coli、Staphylococcus aureus an...
出处
《中国实验方剂学杂志》
CAS
2008年第5期4-6,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
教育部科学技术研究重点项目(206066)
安徽省自然科学基金项目资助项目(050430501)
高等学校博士点专项科研基金(20060370001)
