摘要
目的:比较中药方灌肠Ⅰ号与西药柳氮磺胺吡啶(SASP)对2,4-二硝基氯苯(DNCB)和乙酸(AA)复合法诱导的小鼠溃疡性结肠炎(UC)模型的治疗效果,并探寻UC发病机制。方法:将小鼠随机分为正常组、模型组、SASP组及灌肠Ⅰ号治疗组4组,用肠道积分法评定组织学变化;用流式细胞仪检测各组外周血、肠系膜淋巴结中CD3+、CD4+T细胞的变化。结果:模型组小鼠肠道组织学评分明显高于正常组,而结肠长度明显较正常组缩短;模型组CD3+、CD4+细胞较正常组明显降低(P<0.01)。经SASP和灌肠Ⅰ号灌肠治疗后,在肠道炎症和病理损伤明显减轻的同时,肠道组织学评分较模型组明显降低(P<0.01),结肠长度与正常组基本接近,且CD3+、CD4+细胞亚群较模型组明显增高(P<0.01)。结论:灌肠Ⅰ号对DNCB-AA诱导的小鼠UC模型治疗取得了满意效果,可作为UC发病机制的研究及治疗的较理想药物。
Objective:To compare the therapeutic effects of Clyster No.1(CN1,the Pulsatillae decoction) with salazosulfapyridine(SASP) to the ulcerative colitis(UC) model induced by dinitrochlorobenzene (DNCB)-acetic acid(AA) in mice,to research the pathogenesis.Methods:KM mice were randomly divided into four groups: normal control group,experimental colitis model group,SASP group and CN1 therapeutic group.Proportions of CD3^+、CD4^+ T cell、 in mesenteric lymph node and peripheral blood of each group were estimated by f...
出处
《中国实验方剂学杂志》
CAS
2008年第7期63-66,共4页
Chinese Journal of Experimental Traditional Medical Formulae
