摘要
目的:采用数字序列相关性分析法对灯盏花素的HPLC和UV测定法进行相关性研究。方法:分别用HPLC法测定灯盏花素中野黄芩甙含量和UV法测定灯盏花素中黄酮苷含量,再采用数字序列相关性分析法对两种测定方法的相关性进行统计分析。结果:通过数字序列相关性分析法,得到Cμ1.μ2=0.9993;r=0.7569,说明灯盏花素的两种含量测定方法具有高度相关性,进一步可拟合出它们之间的线性方程:y=-0.4320+1.4311x,r=0.9055>r(3)0.05=0.878,P<0.05。结论:用于测定灯盏花素含量的HPLC法和UV法具有良好的相关性,通过转换系数可由一种含量测定数据推测出另一方法的结果,即可以简化含量测定方法。
Objective:To study different assaysments of Breviscapinun.Method:Breviscapinun content was assayed both by HPLC and UV,and related digital sequences was compaired through correlation analysis.Result:validation results with the correlating the digital sequences with each other,showed that two methods correlated well with a r= 0.905 5.Conclusion:Two methods correlated well.
出处
《中国实验方剂学杂志》
CAS
2008年第8期10-12,共3页
Chinese Journal of Experimental Traditional Medical Formulae
