摘要
目的:探讨结肠及腹膜返折以上直肠癌组织核转录因子(NF-κB)、基质金属蛋白酶-9(MMP-9)蛋白表达与腹腔微转移的关系。方法:收集结肠癌和腹膜返折以上的直肠癌患者80例腹腔冲洗液,进行CEA、CK20免疫细胞化学染色确定腹腔微转移;采用免疫组织化学法检测原发肿瘤NF-κB、MMP-9蛋白表达情况;以透射电镜观察癌细胞之间的连接特点。结果:1)CEA、CK20联合检测的腹腔微转移率为35.0%(28/80)。2)结直肠癌组织中NF-κB、MMP-9表达阳性率均明显高于正常黏膜,P<0.05;NF-κB阳性组和阴性表达组中腹腔微转移的检出率分别为40.7%(24/59)和19.0%(4/21),P<0.05。MMP-9阳性表达和阴性表达组腹腔微转移的检出率分别为39.4%(26/66)和14.3%(2/14),P<0.05;NF-κB与MMP-9之间不仅存在相关关系且为线性关系。3)腹腔微转移阳性患者的肿瘤细胞之间的连接以简单连接和接触连接为主,而阴性组细胞间存在较多的桥粒连接。结论:NF-κB、MMP-9可能参与了结直肠癌腹腔微转移。NF-κB可能通过上调MMP-9表达,而促进癌细胞脱落至腹腔形成微转移。
OBJECTIVE:To investigate the significance of NF-κB and MMPs expressions in peritoneal micrometastases of colorectal carcinoma.METHODS:Eighty samples of colorectal cancers were studied.CEA and CK20 expressions of cancer cells in peritoneal lavage were detected by immunocytochemistry.The expressions of NF-κB and MMP-9 of primary tumors were detected by Immunohistochemistry technology.A transmission electronic microscope(TEM)was used to observe the cell junction feature of colorectal carcinoma.RESULTS:1)The po...
出处
《中华肿瘤防治杂志》
CAS
2008年第13期1014-1017,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
河北省科技攻关课题(07109)
关键词
结直肠肿瘤
腹腔
肿瘤转移
明胶酶B
colorectal neoplasms
abdominal cavity
neoplasm metastasis
gelatinase B
