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马立克氏病病毒类白细胞介素8(vIL-8)的生物趋化作用 被引量：3

Biological chemotaxis of vIL-8 protein encoded by Marek's disease virus
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摘要 利用杆状病毒表达系统,将病毒源性趋化因子病毒类白细胞介素8(vIL-8)基因克隆入转移载体pFastBacI,然后转化到DH10Bac感受态细胞中与Bacmid杆状病毒穿梭载体进行转座重组,通过重组子转染Sf9细胞得到含vIL-8的重组病毒rBacvIL-8。间接免疫荧光试验和雏鸡T、B淋巴细胞生物趋化分析结果表明,vIL-8在昆虫细胞中得到了表达,重组vIL-8在一定浓度范围内,可使鸡T、B细胞发生趋化作用,最高趋化指数分别为3.31±0.50和4.02±0.48,且是典型剂量依赖的钟形趋化性反应曲线;进一步分析发现vIL-8对B细胞趋化活性较对T细胞强?P<0.05),提示vIL-8对靶细胞的趋化性与病毒感染过程相关,在早期溶B细胞感染中有重要作用。 In this study,vIL-8 gene was cloned into pFastBacI to get the recombinant baculovirus shuttle vector(Bacmid-vIL-8)by transforming DH10Bac competent cells.Then Sf9 insect cells were transfected with the recombinant Bacmid-vIL-8 DNA.Finally the recombinant virus that contained vIL-8 gene was obtained.The recombinant products of vIL-8 in IFA were expressed in Sf9 cells infected with the recombinant virus.Our results showed that the recombinant vIL-8 was able to attract chicken T and B lymphocytes in chemotaxis...
作者 高巍 秦爱建 张鑫宇 金文杰 邵红霞
机构地区 扬州大学江苏省动物预防医学重点实验室
出处 《中国兽医学报》 CAS CSCD 北大核心 2008年第6期623-626,共4页 Chinese Journal of Veterinary Science
基金 国家自然科学基金资助项目(30270982) 高等学校全国优秀博士学位论文作者专项资助基金项目(200256)
关键词 马立克氏病病毒 vIL-8 杆状病毒 趋化活性 MDV vIL-8 pathogenesis baculovirus chemotaxis T cells B cells
分类号 S855 [农业科学—临床兽医学]
  • 相关文献

参考文献9

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二级参考文献23

共引文献6

同被引文献28

  • 1李迎晓,秦爱建,刘红梅,邵红霞,金文杰,刘岳龙.鸡传染性腔上囊病病毒VP2基因在昆虫细胞中的表达[J].中国兽医科技,2005,35(12):933-936. 被引量：6
  • 2王健,赵金红.趋化因子受体研究进展[J].现代预防医学,2006,33(2):165-167. 被引量：14
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引证文献3

二级引证文献6

中国兽医学报
2008年 第6期

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