摘要
选择同处于泌乳初期的睢宁白山羊6头,体质量30~40 kg,C.M.T.法检测每头山羊2个乳区乳的体细胞数(SCC)均低于5×105/mL。每头山羊每天早上挤奶后,于右乳区经乳头管灌注黄芪多糖(APS)5 mL,左乳区则通过乳头管灌注等量生理盐水(PSS)作为对照,连续灌注5 d。第6天早上挤奶后,按50μg/kg剂量于左、右乳区通过乳头管注入大肠杆菌内毒素,分别于内毒素处理前1 h及处理后1、2、3、4、5、6、7、8、9、24 h观察乳房的变化并记录直肠温度;并于内毒素处理前1 h、处理后3、6、9、24 h取颈静脉血和采集左、右2个乳区的乳样,采用放射免疫法测定乳汁及血清中TNF-α和IL-2的水平;内毒素处理后24 h,处死动物,取乳腺组织,Bouin氏液固定,H.E.染色进行病理组织学观察。临床症状与病理切片观察均显示,通过乳导管将50μg/kg的内毒素灌注到山羊的乳腺内迅速诱导出典型的急性乳腺炎症状。灌注APS的乳区,乳中TNF-α的水平内毒素处理后3 h显著高于灌注PSS的乳区,92、4 h显著低于灌注PSS的乳区(P<0.05)。与内毒素处理前相比,灌注APS的乳区在内毒素处理后,乳中IL-2...
Six Suining white goats in their early lactation,weighing 30-40 kg were used in this study.Before experiment,total quarter milk SCC were measured with California Mastitis Test(C.M.T.).The individual quarter SCC were less than 500 000 cells/mL.The mammary right quarters of 6 goats were infused with about 5 mL of APS.Left quarters were used as controls and infused with 5 mL of sterile saline.The administration of APS had been continuing for five consecutive days.On the sixth day,after morning milking,the mamm...
出处
《中国兽医学报》
CAS
CSCD
北大核心
2008年第7期844-847,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30371049)
河南省杰出人才创新基金资助项目(0121000300)
关键词
黄芪多糖
山羊
乳腺炎
内毒素
细胞因子
astragalus polysaccharide
goat
mastitis
endotoxin
cytokines
